Evaluation of endogenous reference genes for analysis of gene expression with real-time RT-PCR during planarian regeneration
Evaluation of endogenous reference genes for analysis of gene expression with real-time RT-PCR during planarian regeneration
复制标题
真涡虫再生过程中实时 RT-PCR 分析基因表达的内源参考基因评估
DOI：
10.1007/s11033-010-0570-8
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发表时间：
2011-10
影响因子：
2.8
通讯作者：
Xiao-juan Sun
中科院分区：
文献类型：
--
作者：
Zi-mei Dong;Yan-qing Yuwen;Qing-hua Wang;Guang-wen Chen;Chang-ying Shi;Xiao-juan Sun
It is important that endogenous reference genes for real-time RT-PCR be empirically evaluated for stability in different cell types, developmental stages, and/or sample treatment. To select the most stable endogenous reference genes during planarian regeneration, three housekeeping genes,18S rRNA,ACTBandDjEF2, were identified and established expression levels by real-time RT-PCR. The data were analyzed by GeNorm and NormFinder software. Expression levels of theDjsix-1gene were studied in parallel withACTBandDjEF2both or each and18S rRNAas reference during regeneration. The results showed thatACTBwas the most stable expressed reference gene in the planarian regeneration.
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影响因子：
1.8
作者：
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通讯作者：
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2.9
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Gonzalez-Verdejo, C. I.;Die, J. V.;Roman, B.
通讯作者：
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影响因子：
2.8
作者：
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