Quantitative Correlation between the protein primary sequences and secondary structures in spider dragline silks.
Quantitative Correlation between the protein primary sequences and secondary structures in spider dragline silks.
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DOI：
10.1021/bm9010672
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发表时间：
2010-01-11
影响因子：
6.2
通讯作者：
Yarger, Jeffery L.
中科院分区：
文献类型：
--
作者：
Jenkins, Janelle E.;Creager, Melinda S.;Lewis, Randolph V.;Holland, Gregory P.;Yarger, Jeffery L.
合成蜘蛛丝在从医疗用途到超轻型装甲的各种应用中具有巨大的潜力，然而，生产具有与天然蜘蛛丝相当的机械性能的合成纤维一直困扰着科学界。天然拖丝蜘蛛丝通常由含有高度重复的氨基酸基序的蛋白质制成，采用一系列二级结构。在基于蜘蛛丝蛋白的合成纤维生产取得进一步进展之前，必须知道蛋白质中形成特定二级结构的每种氨基酸的百分比。将这些百分比与蛋白质的一级氨基酸序列联系起来，将建立合成丝的结构基础。在这项研究中，核磁共振（NMR）技术被用来量化的百分比的丙氨酸，甘氨酸，丝氨酸形成β-折叠和螺旋二级结构。这三种氨基酸的分数和它们的二级结构定量地与包含来自棒足环蛛的大壶腹丝和小壶腹丝的蛋白质的一级氨基酸序列相关，提供了合成蜘蛛丝的蓝图。
Synthetic spider silk holds great potential for use in various applications spanning medical uses to ultra lightweight armor, however producing synthetic fibers with mechanical properties comparable to natural spider silk has eluded the scientific community. Natural dragline spider silks are commonly made from proteins that contain highly repetitive amino acid motifs, adopting an array of secondary structures. Before further advances can be made in the production of synthetic fibers based on spider silk proteins, it is imperative to know the percentage of each amino acid in the protein that forms a specific secondary structure. Linking these percentages to the primary amino acid sequence of the protein will establish a structural foundation for synthetic silk. In this study, Nuclear Magnetic Resonance (NMR) techniques are used to quantify the percentage of Ala, Gly, and Ser that form both β-sheet and helical secondary structures. The fraction of these three amino acids and their secondary structure are quantitatively correlated to the primary amino acid sequence for the proteins that comprise major and minor ampullate silk from the Nephila clavipes spider providing a blueprint for synthetic spider silks.
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影响因子：
5.5
作者：
Grubb, DT;Jelinski, LW
通讯作者：
Jelinski, LW
DOI：
10.1039/b812928b
发表时间：
2008-11-21
期刊：
Chemical communications (Cambridge, England)
影响因子：
--
作者：
Holland GP;Jenkins JE;Creager MS;Lewis RV;Yarger JL
通讯作者：
Yarger JL
影响因子：
56.9
作者：
Lazaris, A;Arcidiacono, S;Karatzas, CN
通讯作者：
Karatzas, CN
影响因子：
6.2
作者：
Brooks, Amanda E.;Stricker, Shane M.;Lewis, Randolph V.
通讯作者：
Lewis, Randolph V.
影响因子：
5.6
作者：
Asakura, T;Ashida, J;Komatsu, K
通讯作者：
Komatsu, K