Bacterial communities of the coronal sulcus and distal urethra of adolescent males.
Bacterial communities of the coronal sulcus and distal urethra of adolescent males.
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DOI：
10.1371/journal.pone.0036298
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Fortenberry JD
中科院分区：
文献类型：
--
作者：
Nelson DE;Dong Q;Van der Pol B;Toh E;Fan B;Katz BP;Mi D;Rong R;Weinstock GM;Sodergren E;Fortenberry JD
以乳酸杆菌为主的阴道微生物菌群与妇女的生殖健康和性传播感染抵抗有关，而改变的微生物菌群与细菌性阴道病（BV）、性传播感染风险和不良生殖结果有关。在男性首采尿、尿道拭子和冠状沟（CS）标本中观察到了推定的阴道分类群，但这些观察结果的意义尚不清楚。我们使用16 S rRNA测序来表征连续三个月从18名青少年男性收集的CS和尿液的微生物群。大多数参与者的CS微生物比他们的尿液微生物更稳定，并且CS微生物的组成受到包皮环切术的强烈影响。BV相关的类群，包括Atopobium，Megaspaera，Mobiluncus，Prevotella和Gemella，检测CS标本从性经验丰富的和缺乏经验的参与者。相比之下，尿液中主要含有CS标本中不丰富的分类群。乳酸杆菌和链球菌是主要的尿液类群，但它们的丰度呈负相关。相比之下，Sneathia，支原体和支原体只在性活跃参与者的尿液中发现。因此，CS和尿液支持稳定且独特的细菌群落。最后，我们的研究结果表明，阴茎和尿道可以由各种BV相关的类群，这些殖民地的一些结果从合作伙伴的性活动。
Lactobacillus-dominated vaginal microbiotas are associated with reproductive health and STI resistance in women, whereas altered microbiotas are associated with bacterial vaginosis (BV), STI risk and poor reproductive outcomes. Putative vaginal taxa have been observed in male first-catch urine, urethral swab and coronal sulcus (CS) specimens but the significance of these observations is unclear. We used 16 S rRNA sequencing to characterize the microbiota of the CS and urine collected from 18 adolescent men over three consecutive months. CS microbiotas of most participants were more stable than their urine microbiotas and the composition of CS microbiotas were strongly influenced by circumcision. BV-associated taxa, including Atopobium, Megasphaera, Mobiluncus, Prevotella and Gemella, were detected in CS specimens from sexually experienced and inexperienced participants. In contrast, urine primarily contained taxa that were not abundant in CS specimens. Lactobacilllus and Streptococcus were major urine taxa but their abundance was inversely correlated. In contrast, Sneathia, Mycoplasma and Ureaplasma were only found in urine from sexually active participants. Thus, the CS and urine support stable and distinct bacterial communities. Finally, our results suggest that the penis and the urethra can be colonized by a variety of BV-associated taxa and that some of these colonizations result from partnered sexual activity.
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影响因子：
3.7
作者：
Price LB;Liu CM;Johnson KE;Aziz M;Lau MK;Bowers J;Ravel J;Keim PS;Serwadda D;Wawer MJ;Gray RH
通讯作者：
Gray RH
影响因子：
2.1
作者：
Atassi, Fabrice;Servin, Alain L.
通讯作者：
Servin, Alain L.
影响因子：
4.4
作者：
Harwich, Michael D., Jr.;Alves, Joao M.;Jefferson, Kimberly K.
通讯作者：
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影响因子：
5.8
作者：
Huber, T;Faulkner, G;Hugenholtz, P
通讯作者：
Hugenholtz, P
影响因子：
12.3
作者：
Lennon NJ;Lintner RE;Anderson S;Alvarez P;Barry A;Brockman W;Daza R;Erlich RL;Giannoukos G;Green L;Hollinger A;Hoover CA;Jaffe DB;Juhn F;McCarthy D;Perrin D;Ponchner K;Powers TL;Rizzolo K;Robbins D;Ryan E;Russ C;Sparrow T;Stalker J;Steelman S;Weiand M;Zimmer A;Henn MR;Nusbaum C;Nicol R
通讯作者：
Nicol R