Plk1 is essential for proper chromosome segregation during meiosis I/meiosis II transition in pig oocytes
Plk1 is essential for proper chromosome segregation during meiosis I/meiosis II transition in pig oocytes
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Plk1 对于猪卵母细胞减数分裂 I/减数分裂 II 过渡过程中正确的染色体分离至关重要
DOI：
10.1186/s12958-017-0289-7
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发表时间：
2017-08
影响因子：
4.4
通讯作者：
Ju Shiqiang
中科院分区：
文献类型：
--
作者：
Zhang Zixiao;Chen Changchao;Ma Liying;Yu Qiuchen;Li Shuai;Abbasi Benazir;Yang Jiayi;Rui Rong;Ju Shiqiang
研究背景Polo样激酶1（Plk 1）作为减数分裂的特征性调控因子，参与细胞分裂的多个生物学事件。虽然Plk 1已牵连在体细胞有丝分裂过程中的各种功能，相当少的是已知的关于其功能在从中期I（MI）到中期II（MII）阶段在卵母细胞减数分裂progress.MethodsIn这项研究中，Plk 1在MI到MII阶段过渡猪卵母细胞的可能作用。首先，采用间接免疫荧光和共聚焦显微成像技术结合Western blot分析，揭示了Plk 1在猪MI至MII期卵母细胞中的时空表达和亚细胞定位模式。此外，一个高选择性的Plk 1抑制剂，GSK 461364，被用来确定Plk 1在这个MI到MII的过渡progress.ResultsPlk1的潜在作用表现出特定的动态细胞内定位，和共定位的Plk 1与α-微管蛋白被揭示在猪卵母细胞减数分裂纺锤体从MI到MII阶段的过渡。GSK 461364处理以剂量依赖性方式显著阻断第一极体（pbI）发射，并导致减数分裂成熟失败，其中较大百分比的GSK 461364处理的卵母细胞停滞在后期-末期I（ATI）阶段。进一步的亚细胞结构检查结果表明，用GSK 461364抑制Plk 1对纺锤体组装没有明显影响，但使处理的卵母细胞中有显著比例的卵母细胞在ATI期同源染色体分离中有明显缺陷。这种调控与Plk 1对ATI期同源染色体分离的影响有关。
BackgroundPolo-like kinase 1 (Plk1), as a characteristic regulator in meiosis, organizes multiple biological events of cell division. Although Plk1 has been implicated in various functions in somatic cell mitotic processes, considerably less is known regarding its function during the transition from metaphase I (MI) to metaphase II (MII) stage in oocyte meiotic progression.MethodsIn this study, the possible role of Plk1 during the MI-to-MII stage transition in pig oocytes was addressed. Initially, the spatiotemporal expression and subcellular localization pattern of Plk1 were revealed in pig oocytes from MI to MII stage using indirect immunofluorescence and confocal microscopy imaging techniques combined with western blot analyses. Moreover, a highly selective Plk1 inhibitor, GSK461364, was used to determine the potential role of Plk1 during this MI-to-MII transition progression.ResultsUpon expression, Plk1 exhibited a specific dynamic intracellular localization, and co-localization of Plk1 with α-tubulin was revealed in the meiotic spindle of pig oocyte during the transition from MI to MII stage. GSK461364 treatment significantly blocked the first polar body (pbI) emission in a dose-dependent manner and resulted in a failure of meiotic maturation, with a larger percentage of the GSK461364-treated oocytes arresting in the anaphase-telophase I (ATI) stage. Further subcellular structure examination results showed that inhibition of Plk1 with GSK461364 had no visible effect on spindle assembly but caused a significantly higher proportion of the treated oocytes to have obvious defects in homologous chromosome segregation at ATI stage.ConclusionsThus, these results indicate that Plk1 plays an essential role during the meiosis I/meiosis II transition in porcine oocytes, and the regulation is associated with Plk1’s effects on homologous chromosome segregation in the ATI stage.
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影响因子：
112.7
作者：
Vincent Archambault;D. Glover
通讯作者：
Vincent Archambault;D. Glover
影响因子：
3.8
作者：
Ma, W;Hou, Y;Wang, WH
通讯作者：
Wang, WH
影响因子：
3.7
作者：
Brennan IM;Peters U;Kapoor TM;Straight AF
通讯作者：
Straight AF
小鼠卵母细胞成熟过程中 PLK1 的多种需求。
影响因子：
3.7
作者：
Solc P;Kitajima TS;Yoshida S;Brzakova A;Kaido M;Baran V;Mayer A;Samalova P;Motlik J;Ellenberg J
通讯作者：
Ellenberg J
影响因子：
4.3
作者：
Bo Xiong;Shao‐Chen Sun;Sheng-Li Lin;Mo Li;Bao-Zeng Xu;Yingchun Ouyang;Yi Hou;Dayuan Chen;Qing-Yuan Sun
通讯作者：
Bo Xiong;Shao‐Chen Sun;Sheng-Li Lin;Mo Li;Bao-Zeng Xu;Yingchun Ouyang;Yi Hou;Dayuan Chen;Qing-Yuan Sun