Comparative study of allogenic and xenogeneic mesenchymal stem cells on cisplatin-induced acute kidney injury in Sprague-Dawley rats.
Comparative study of allogenic and xenogeneic mesenchymal stem cells on cisplatin-induced acute kidney injury in Sprague-Dawley rats.
复制标题
DOI：
10.1186/s13287-016-0386-0
复制
发表时间：
2016-09-01
影响因子：
7.5
通讯作者：
Sobh M
中科院分区：
文献类型：
--
作者：
Ashour RH;Saad MA;Sobh MA;Al-Husseiny F;Abouelkheir M;Awad A;Elghannam D;Abdel-Ghaffar H;Sobh M
间充质干细胞（MSC）的旁分泌和再生活性可能因干细胞来源的不同而异。本研究的目的是比较不同来源的间充质干细胞对顺铂诱导的急性肾损伤（AKI）的作用及其对肾再生的影响。在 120 只 Sprague-Dawley 大鼠中单次腹腔注射顺铂 (5 mg/kg) 诱导 AKI。用大鼠骨髓干细胞（rBMSCs）、人脂肪组织源性干细胞（hADSCs）或人羊水源性干细胞（hAFSCs）治疗大鼠。注射顺铂24小时后，通过大鼠尾静脉单次注射5 × 106个不同来源的MSC。每组中，在注射顺铂后第4、7、11和30天处死大鼠。测量血清肌酐、BUN 和肾组织氧化应激参数。对肾组织进行组织病理学评分，以获得损伤、再生和慢性的证据。还使用 Ki67 进行免疫组织化学评估肾增殖活性。三种来源的间充质干细胞能够改善顺铂引起的肾功能恶化和组织损伤。与 hADSC 和 hAFSC 相比，大鼠 BMSC 治疗组在第 30 天时血清肌酐最低（0.52±0.06）。所有 MSC 治疗组都具有几乎相同的抗氧化活性，如不同时间间隔的肾组织丙二醛 (MDA) 降低、还原型谷胱甘肽 (GSH) 水平和超氧化物歧化酶 (SOD) 活性增加。此外，所有 MSC 均改善了损伤和再生评分。 Ki67 鉴定，大鼠 BMSC 在第 7 天时在肾皮质中具有最高计数和最早的增殖活性；而 hAFSC 的再生和增殖活性似乎有最大的改善，第 11 天肾皮质 Ki67 阳性细胞计数更高，坏死病变最少。大鼠 BMSC、hADSC 和 hAFSC 在早期单次静脉注射中对顺铂诱导的 AKI 具有肾脏保护作用，并且能够减少氧化应激标志物。大鼠 BMSC 在第 7 天出现最早的增殖活性；然而，hAFSCs 的再生活性似乎有最大的改善。人类 ADSC 在增殖和再生活性方面效率最低。
The paracrine and regenerative activities of mesenchymal stem cells (MSCs) may vary with different stem cell sources. The aim of the present study is to compare the effects of MSCs from different sources on acute kidney injury (AKI) induced by cisplatin and their influence on renal regeneration. A single intraperitoneal injection of cisplatin (5 mg/kg) was used to induce AKI in 120 Sprague-Dawley rats. Rats were treated with either rat bone marrow stem cells (rBMSCs), human adipose tissue-derived stem cells (hADSCs), or human amniotic fluid-derived stem cells (hAFSCs). 5 × 106 MSCs of different sources were administered through rat tail vein in a single dose, 24 hours after cisplatin injection. Within each group, rats were sacrificed at the 4th, 7th, 11th, and 30th day after cisplatin injection. Serum creatinine, BUN, and renal tissue oxidative stress parameters were measured. Renal tissue was scored histopathologically for evidence of injury, regeneration, and chronicity. Immunohistochemistry was also done using Ki67 for renal proliferative activity evaluation. MSCs of the three sources were able to ameliorate cisplatin-induced renal function deterioration and tissue damage. The rat BMSCs-treated group had the lowest serum creatinine by day 30 (0.52 ± 0.06) compared to hADSCs and hAFSCs. All MSC-treated groups had nearly equal antioxidant activity as indicated by the decreased renal tissue malondialdehyde (MDA) and increased reduced glutathione (GSH) level and superoxide dismutase (SOD) activity at different time intervals. Additionally, all MSCs improved injury and regenerative scores. Rat BMSCs had the highest count and earliest proliferative activity in the renal cortex by day 7 as identified by Ki67; while, hAFSCs seem to have the greatest improvement in the regenerative and proliferative activities with a higher count of renal cortex Ki67-positive cells at day 11 and with the least necrotic lesions. Rat BMSCs, hADSCs, and hAFSCs, in early single IV dose, had a renoprotective effect against cisplatin-induced AKI, and were able to reduce oxidative stress markers. Rat BMSCs had the earliest proliferative activity by day 7; however, hAFSCs seemed to have the greatest improvement in the regenerative activities. Human ADSCs were the least effective in the terms of proliferative and regenerative activities.
登录
查看更多内容
影响因子：
--
作者：
Kretlow JD;Jin YQ;Liu W;Zhang WJ;Hong TH;Zhou G;Baggett LS;Mikos AG;Cao Y
通讯作者：
Cao Y
影响因子：
5.6
作者：
Jin, Hye Jin;Bae, Yun Kyung;Chang, Jong Wook
通讯作者：
Chang, Jong Wook
影响因子：
5.2
作者：
Kern, Susanne;Eichler, Hermann;Bieback, Karen
通讯作者：
Bieback, Karen
影响因子：
2.7
作者：
Collins, Tony J.
通讯作者：
Collins, Tony J.
影响因子：
19.6
作者：
van Roeyen, CRC;Ostendorf, T;Floege, J
通讯作者：
Floege, J