Adhesion of Enteropathogenic, Enterotoxigenic, and Commensal Escherichia coli to the Major Zymogen Granule Membrane Glycoprotein 2.
Adhesion of Enteropathogenic, Enterotoxigenic, and Commensal Escherichia coli to the Major Zymogen Granule Membrane Glycoprotein 2.
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DOI：
10.1128/aem.02279-21
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发表时间：
2022-03-08
影响因子：
4.4
通讯作者：
Schierack P
中科院分区：
文献类型：
--
作者：
Bartlitz C;Kolenda R;Chilimoniuk J;Grzymajło K;Rödiger S;Bauerfeind R;Ali A;Tchesnokova V;Roggenbuck D;Schierack P
致病菌如肠致病性大肠杆菌（EPEC）和肠致病性大肠杆菌（EAE）等。大肠杆菌（ETEC），导致哺乳动物腹泻。特别是E.大肠杆菌通过1型菌毛（T1 F）定殖并感染胃肠道。在此，主要酶原颗粒膜糖蛋白2（GP 2）充当宿主细胞受体。GP 2也由胰腺和各种粘液腺分泌，与管腔1型菌毛阳性E.杆菌目前尚不清楚GP 2亚型是否具有特异性E。大肠杆菌致病型结合。在本研究中，我们研究了人、猪和牛的EPEC和ETEC以及大肠杆菌的相互作用。大肠杆菌分离株与人、猪和牛GP 2的杂交。我们首先定义了致病型和宿主相关的FimH变体。其次，我们可以证明GP 2亚型与FimH变体在不同程度上结合。然而，GP 2-FimH相互作用似乎不受E.杆菌相反，可溶性GP 2影响ETEC感染和巨噬细胞的吞噬率。ETEC致病型与GP 2的预孵育减少了细胞系的感染。此外，E. GP 2能提高巨噬细胞的吞噬率。我们的研究结果表明，GP 2在防御E.大肠杆菌感染并产生相应的宿主免疫应答。重要性病原菌的感染，如某些大肠杆菌致病型，导致哺乳动物腹泻。病原体，包括人畜共患病病原体，可以感染不同的宿主或显示宿主特异性。有些大肠杆菌菌株经常在人类和动物之间传播，而其他大肠杆菌菌株倾向于只在一个宿主中定植。这种宿主特异性仍然没有完全理解。我们表明，糖蛋白2是一个选择性受体，特定的大肠杆菌菌株或变种的粘附素FimH，但不是一个选择器的物种特异性大肠杆菌组。我们证明GP 2参与了定植和感染的调节，因此代表了用于预防或治疗疾病的感兴趣的分子。
Pathogenic bacteria, such as enteropathogenic Escherichia coli (EPEC) and enterotoxigenic E. coli (ETEC), cause diarrhea in mammals. In particular, E. coli colonizes and infects the gastrointestinal tract via type 1 fimbriae (T1F). Here, the major zymogen granule membrane glycoprotein 2 (GP2) acts as a host cell receptor. GP2 is also secreted by the pancreas and various mucous glands, interacting with luminal type 1 fimbriae-positive E. coli. It is unknown whether GP2 isoforms demonstrate specific E. coli pathotype binding. In this study, we investigated interactions of human, porcine, and bovine EPEC and ETEC, as well as commensal E. coli isolates with human, porcine, and bovine GP2. We first defined pathotype- and host-associated FimH variants. Second, we could prove that GP2 isoforms bound to FimH variants to various degrees. However, the GP2-FimH interactions did not seem to be influenced by the host specificity of E. coli. In contrast, soluble GP2 affected ETEC infection and phagocytosis rates of macrophages. Preincubation of the ETEC pathotype with GP2 reduced the infection of cell lines. Furthermore, preincubation of E. coli with GP2 improved the phagocytosis rate of macrophages. Our findings suggest that GP2 plays a role in the defense against E. coli infection and in the corresponding host immune response. IMPORTANCE Infection by pathogenic bacteria, such as certain Escherichia coli pathotypes, results in diarrhea in mammals. Pathogens, including zoonotic agents, can infect different hosts or show host specificity. There are Escherichia coli strains which are frequently transmitted between humans and animals, whereas other Escherichia coli strains tend to colonize only one host. This host specificity is still not fully understood. We show that glycoprotein 2 is a selective receptor for particular Escherichia coli strains or variants of the adhesin FimH but not a selector for a species-specific Escherichia coli group. We demonstrate that GP2 is involved in the regulation of colonization and infection and thus represents a molecule of interest for the prevention or treatment of disease.
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影响因子：
4.3
作者：
Hoelzl, Markus A.;Hofer, Johannes;Kovarik, Johannes J.;Roggenbuck, Dirk;Reinhold, Dirk;Goihl, Alexander;Gaertner, Miriam;Steinberger, Peter;Zlabinger, Gerhard J.
通讯作者：
Zlabinger, Gerhard J.
影响因子：
6.7
作者：
Dreux N;Denizot J;Martinez-Medina M;Mellmann A;Billig M;Kisiela D;Chattopadhyay S;Sokurenko E;Neut C;Gower-Rousseau C;Colombel JF;Bonnet R;Darfeuille-Michaud A;Barnich N
通讯作者：
Barnich N
影响因子：
2.9
作者：
BLUM, H;BEIER, H;GROSS, HJ
通讯作者：
GROSS, HJ
影响因子：
3.6
作者：
Hung, CS;Bouckaert, J;Hultgren, SJ
通讯作者：
Hultgren, SJ
DOI：
10.1016/s0167-4781(00)00057-9
发表时间：
2000-04-25
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
影响因子：
--
作者：
Fukuoka, SI
通讯作者：
Fukuoka, SI