Self-assembly of globular proteins with intrinsically disordered protein polyelectrolytes and block copolymers.
Self-assembly of globular proteins with intrinsically disordered protein polyelectrolytes and block copolymers.
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无序多肽是一类用途广泛的材料，它将多肽的生物相容性与合成聚合物的无序结构和多样的相行为相结合。当与带相反电荷的聚电解质混合时，合成聚电解质能够具有复杂的相行为，促进纳米颗粒形成和本体相分离。然而，已经有有限的探索本质上无序的蛋白质聚电解质作为合成聚电解质的潜在生物基替代品。在这里，我们生产带负电荷的，内在无序的多肽，能够在大肠杆菌中高产量表达。大肠杆菌，并使用这种内在无序的肽来产生完全基于蛋白质的复合物。配合物显示丰富的相行为，显示电荷密度，盐浓度，温度和电荷分数的敏感性。我们通过浊度测定，动态光散射和透射电子显微镜的组合来表征这种行为。固有无序肽的稳健表达谱和刺激响应相行为证明了它们作为合成聚电解质的容易生产的生物相容性替代品的潜力。
Intrinsically disordered polypeptides are a versatile class of materials, combining the biocompatibility of peptides with the disordered structure and diverse phase behaviors of synthetic polymers. Synthetic polyelectrolytes are capable of complex phase behavior when mixed with oppositely charged polyelectrolytes, facilitating nanoparticle formation and bulk phase separation. However, there has been limited exploration of intrinsically disordered protein polyelectrolytes as potential bio-based replacements for synthetic polyelectrolytes. Here, we produce negatively charged, intrinsically disordered polypeptides, capable of high-yield expression in E. coli and use this intrinsically disordered peptide to produce entirely protein-based polyelectrolyte complexes. The complexes display rich phase behavior, showing sensitivity to charge density, salt concentration, temperature, and charge fraction. We characterize this behavior through a combination of turbidity assays, dynamic light scattering, and transmission electron microscopy. The robust expression profile and stimuli-responsive phase behavior of the intrinsically disordered peptides demonstrates their potential as easily producible, biocompatible substitutes for synthetic polyelectrolytes.
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具有可切换表面电荷的光响应聚离子复合胶束，可实现高效蛋白质递送
影响因子：
7.015
作者：
Jin, Qiao;Cai, Tongjiang;Ji, Jian
通讯作者：
Ji, Jian
影响因子：
15
作者：
Klass, Sarah H.;Smith, Matthew J.;Francis, Matthew B.
通讯作者：
Francis, Matthew B.
影响因子：
12.4
作者：
Akinc, Akin;Goldberg, Michael;Anderson, Daniel G.
通讯作者：
Anderson, Daniel G.
DOI：
10.1038/mt.2011.190
发表时间：
2011-12
期刊：
Molecular therapy : the journal of the American Society of Gene Therapy
影响因子：
--
作者：
通讯作者：
--
NIPAM 和偶氮苯功能化的双响应嵌段共聚物胶束
影响因子：
4.6
作者：
Feng, Ze;Lin, Li;Yu, Yanlei
通讯作者：
Yu, Yanlei