Fluorescence recovery after photobleaching: analyses of cyanobacterial phycobilisomes reveal intrinsic fluorescence recovery
Fluorescence recovery after photobleaching: analyses of cyanobacterial phycobilisomes reveal intrinsic fluorescence recovery
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光漂白后的荧光恢复：蓝藻藻胆体的分析揭示了内在的荧光恢复
DOI：
10.1007/s42995-021-00104-z
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发表时间：
2021-06
影响因子：
5.7
通讯作者：
Zhang Yu-Zhong
中科院分区：
文献类型：
--
作者：
Zhang Nan;Li Kang;Xie Bin-Bin;Chen Xiu-Lan;Zhou Bai-Cheng;Su Hai-Nan;Zhang Yu-Zhong
自1997年以来，光漂白后的荧光恢复（FRAP）一直被用来研究蓝藻光合作用装置的动力学。FRAP期间蓝藻的荧光恢复通常被解释为藻胆体（PBS）在类囊体膜表面扩散的结果。蓝藻的状态转换机制被广泛归因于 PBS 扩散。然而，在红藻（另一种含 PBS 的群体）中，发现内在光过程对 PBS 的荧光恢复有很大贡献，这引发了关于 FRAP 在红藻 PBS 中的作用的问题。因此，重新评估蓝藻中 PBS 荧光恢复的性质非常重要。在本研究中，使用具有不同表型和 PBS 组成的四种蓝藻菌株来研究它们的 FRAP 特性。在所有四种蓝藻菌株的完全光漂白细胞中观察到 PBS 的荧光恢复，其中 PBS 扩散对荧光恢复的贡献是不可能的。此外，光漂白后在分离的 PBS 和 PBS-类囊体膜中恢复的荧光进一步证明了荧光恢复的内在光处理性质。这些发现表明，当通过 FRAP 方法测量时，内在光过程有助于光漂白后的荧光恢复。
Fluorescence recovery after photobleaching (FRAP) has been used to study the dynamics of the cyanobacterial photosynthesis apparatus since 1997. Fluorescence recovery of cyanobacteria during FRAP was conventionally interpreted as a result of phycobilisome (PBS) diffusion on the surface of the thylakoid membrane. The mechanism of state transition in cyanobacteria has been widely attributed to PBS diffusion. However, in red algae, another PBS-containing group, the intrinsic photoprocess was found to contribute greatly to the fluorescence recovery of PBS, which raises questions concerning the role of FRAP in red algal PBS. Therefore, it is important to re-evaluate the nature of PBS fluorescence recovery in cyanobacteria. In the present study, four cyanobacterial strains with different phenotypes and PBS compositions were used to investigate their FRAP characteristics. Fluorescence recovery of PBS was observed in wholly photobleached cells in all four cyanobacterial strains, in which the contribution of PBS diffusion to the fluorescence recovery was not possible. Moreover, the fluorescence recovered in isolated PBSs and PBS-thylakoid membranes after photobleaching further demonstrated the intrinsic photoprocess nature of fluorescence recovery. These findings suggest that the intrinsic photoprocess contributed to the fluorescence recovery following photobleaching when measured by the FRAP method.
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影响因子：
3.7
作者：
Arteni, Ana A.;Liu, Lu-Ning;Aartsma, Thijs J.;Zhang, Yu-Zhong;Zhou, Bai-Cheng;Boekema, Egbert J.
通讯作者：
Boekema, Egbert J.
影响因子：
2.1
作者：
M. Sarcina;C. Mullineaux
通讯作者：
M. Sarcina;C. Mullineaux
影响因子：
3.3
作者：
S. Siebzehnrübl;R. Fischer;W. Kufer;H. Scheer
通讯作者：
S. Siebzehnrübl;R. Fischer;W. Kufer;H. Scheer
DOI：
10.3390/s100605927
发表时间：
2010
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Rayan G;Guet JE;Taulier N;Pincet F;Urbach W
通讯作者：
Urbach W
DOI：
10.1021/j100818a048
发表时间：
1962-12
期刊：
The Journal of Physical Chemistry
影响因子：
--
作者：
R. Livingston;D. Stockman
通讯作者：
R. Livingston;D. Stockman