Recent applications of fluorescence recovery after photobleaching (FRAP) to membrane bio-macromolecules.
Recent applications of fluorescence recovery after photobleaching (FRAP) to membrane bio-macromolecules.
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DOI:
10.3390/s100605927
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发表时间:
2010
期刊:
影响因子:
--
通讯作者:
Urbach W
中科院分区:
文献类型:
--
作者:
Rayan G;Guet JE;Taulier N;Pincet F;Urbach W
This review examines some recent applications of fluorescence recovery after photobleaching (FRAP) to biopolymers, while mainly focusing on membrane protein studies. Initially, we discuss the lateral diffusion of membrane proteins, as measured by FRAP. Then, we talk about the use of FRAP to probe interactions between membrane proteins by obtaining fundamental information such as geometry and stoichiometry of the interacting complex. Afterwards, we discuss some applications of FRAP at the cellular level as well as the level of organisms. We conclude by comparing diffusion coefficients obtained by FRAP and several other alternative methods.
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