Recent applications of fluorescence recovery after photobleaching (FRAP) to membrane bio-macromolecules.

Recent applications of fluorescence recovery after photobleaching (FRAP) to membrane bio-macromolecules.
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DOI:
10.3390/s100605927
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发表时间:
2010
期刊:
Sensors (Basel, Switzerland)
影响因子:
--
通讯作者:
Urbach W
Urbach W
中科院分区:
其他
文献类型:
--
作者:
Rayan G;Guet JE;Taulier N;Pincet F;Urbach W

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本文综述了最近的应用程序的荧光恢复后,光漂白(FRAP)的生物聚合物,而主要集中在膜蛋白的研究。最初,我们讨论膜蛋白的横向扩散,FRAP测量。然后,我们讨论使用FRAP探测膜蛋白之间的相互作用,通过获得基本信息,如几何形状和化学计量的相互作用复合物。随后,我们讨论了FRAP在细胞水平以及生物体水平上的一些应用。最后,我们通过比较FRAP和其他几种方法获得的扩散系数。
This review examines some recent applications of fluorescence recovery after photobleaching (FRAP) to biopolymers, while mainly focusing on membrane protein studies. Initially, we discuss the lateral diffusion of membrane proteins, as measured by FRAP. Then, we talk about the use of FRAP to probe interactions between membrane proteins by obtaining fundamental information such as geometry and stoichiometry of the interacting complex. Afterwards, we discuss some applications of FRAP at the cellular level as well as the level of organisms. We conclude by comparing diffusion coefficients obtained by FRAP and several other alternative methods.
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