Prenatal alcohol exposure enhances the susceptibility to NMDA-induced generalized tonic-clonic seizures in developing rats.
Prenatal alcohol exposure enhances the susceptibility to NMDA-induced generalized tonic-clonic seizures in developing rats.
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DOI：
10.1111/cns.12756
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发表时间：
2017-10
影响因子：
5.5
通讯作者：
N'Gouemo P
中科院分区：
文献类型：
--
作者：
Cho SJ;Lovinger DM;N'Gouemo P
产前酒精暴露（PAE）与婴儿和儿童发生全身强直阵挛性癫痫发作（GTCS）的可能性较高相关。然而，PAE相关癫痫发作的实验研究产生了相互矛盾的结果。在这里，我们研究了急性PAE对N-甲基-D-天冬氨酸（NMDA）诱导的发育中大鼠癫痫发作的影响。妊娠Sprague-Dawley大鼠在胚胎第18天口服乙醇（5 g/kg体重）或溶剂。在出生后第7天（P7）、第21天（P21）、第35天（P35）和第42天（P42）检测后代对NMDA诱导的癫痫发作的敏感性。具体地，记录和分析NMDA诱导的连续疯狂奔跑样行为（CWR）、屈曲发作（FS）、疯狂奔跑发作（WRS）、GTCS和强直性发作（TS）的发生率和潜伏期。 N-甲基-D-天冬氨酸诱导的癫痫发作在<P21大鼠表现为CWR、FS、GTCS和TS，而在>P21大鼠表现为WRS、GTCS和TS。因此，在开发期间始终观察到GTCS。PAE分别显著增加雌性和雄性P7-P21大鼠和P7-P35大鼠中GTCS的患病率，但在老年大鼠中没有。PAE还增加了雄性P21-P35大鼠中TS的患病率，但对雌性P21-P35大鼠无影响。GTCS的PAE动物模型可能提供了一个新的机会，调查的机制，神经元过度兴奋的发育中的动物产前暴露于酒精。
Prenatal alcohol exposure (PAE) is associated with a higher likelihood of developing generalized tonic-clonic seizures (GTCS) in infants and children. However, experimental studies of PAE-related seizures have yielded conflicting results. Here, we investigated the effect of acute PAE on N-methyl-D-aspartate (NMDA)-induced seizures in developing rats. Pregnant Sprague-Dawley rats were given an oral dose of either ethanol (5 g/kg body weight) or vehicle on embryonic day 18. The offspring were tested for susceptibility to NMDA-induced seizures on postnatal day 7 (P7), 21 (P21), 35 (P35) and 42 (P42). Specifically, the prevalence and latency of NMDA-induced continuous wild running-like behaviors (CWR), flexion seizures (FS), wild running seizures (WRS), GTCS, and tonic seizures (TS) were recorded and analyzed. N-methyl-D-aspartate-induced seizures consisted of CWR, FS, GTCS and TS in <P21 rats, while WRS, GTCS and TS were observed in >P21 rats. Thus, GTCS were consistently observed during development. PAE significantly increases the prevalence of GTCS in female and male P7-P21 rats and P7-P35 rats, respectively, but not in older rats. PAE also increases the prevalence of TS in male, but not female P21-P35 rats. The PAE animal model of GTCS may provide a new opportunity to investigate the mechanisms that underlie neuronal hyperexcitability in developing animals prenatally-exposed to alcohol.
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DOI：
10.1111/j.1530-0277.2001.tb02129.x
发表时间：
2001-01-01
影响因子：
3.2
作者：
Bonthius, DJ;Woodhouse, J;Lothman, EW
通讯作者：
Lothman, EW
DOI：
10.3109/15563658909000358
发表时间：
1989-01-01
期刊：
JOURNAL OF TOXICOLOGY-CLINICAL TOXICOLOGY
影响因子：
--
作者：
MINION, GE;SLOVIS, CM;BOUTIETTE, L
通讯作者：
BOUTIETTE, L
DOI：
10.1016/b978-012088554-1/50015-3
发表时间：
2006-01-01
期刊：
MODELS OF SEIZURES AND EPILEPSY
影响因子：
--
作者：
N'Gouemo, Prosper;Rogawski, Michael A.
通讯作者：
Rogawski, Michael A.
影响因子：
10.6
作者：
BROWN, ME;ANTON, RF;BALLENGER, JC
通讯作者：
BALLENGER, JC
影响因子：
2.9
作者：
DRISCOLL, CD;STREISSGUTH, AP;RILEY, EP
通讯作者：
RILEY, EP