Novel putative polyethylene terephthalate (PET) plastic degrading enzymes from the environmental metagenome.
Novel putative polyethylene terephthalate (PET) plastic degrading enzymes from the environmental metagenome.
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文献中描述了几种塑料降解酶，最引人注目的是能够水解聚对苯二甲酸乙二醇酯 (PET) 塑料的 PETase。其中之一是来自 Ideonella sakaiensis 的 PETase，这是一种从 PET 瓶回收场内的环境样本中分离出来的细菌，是广泛研究的主题。为了测试 PETase 功能在其他细菌群落中的广泛性，我们在 JGI 宏基因组数据集中使用了一系列 BLAST 搜索，结果表明，在来自受人类影响和相对原始地点的其他宏基因组环境样本中也可以找到 I. sakaiensis PETase 的密切同源物。为了确认它们被归类为假定的 PETase，我们验证了新鉴定的蛋白质具有已知 PETase 所共有的 PETase 序列特征，并且系统发育分析将它们与实验表征的 PETase 分组。此外，还进行了对接分析，以进一步确认假定的环境 PETase 的功能分配。
Several plastic degrading enzymes have been described in the literature, most notably PETases that are capable of hydrolyzing polyethylene terephthalate (PET) plastic. One of them, the PETase from Ideonella sakaiensis, a bacterium isolated from environmental samples within a PET bottle recycling site, was a subject of extensive studies. To test how widespread PETase functionality is in other bacterial communities, we used a cascade of BLAST searches in the JGI metagenomic datasets and showed that close homologs of I. sakaiensis PETase can also be found in other metagenomic environmental samples from both human-affected and relatively pristine sites. To confirm their classification as putative PETases, we verified that the newly identified proteins have the PETase sequence signatures common to known PETases and that phylogenetic analyses group them with the experimentally characterized PETases. Additionally, docking analysis was performed in order to further confirm the functional assignment of the putative environmental PETases.
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影响因子：
14.9
作者：
Chen IA;Chu K;Palaniappan K;Pillay M;Ratner A;Huang J;Huntemann M;Varghese N;White JR;Seshadri R;Smirnova T;Kirton E;Jungbluth SP;Woyke T;Eloe-Fadrosh EA;Ivanova NN;Kyrpides NC
通讯作者：
Kyrpides NC
影响因子：
14.9
作者：
Trifinopoulos J;Nguyen LT;von Haeseler A;Minh BQ
通讯作者：
Minh BQ
影响因子：
3
作者：
Pettersen, EF;Goddard, TD;Ferrin, TE
通讯作者：
Ferrin, TE
影响因子：
14.9
作者：
Kim, DE;Chivian, D;Baker, D
通讯作者：
Baker, D
对聚乙二醇乙酸乙酯）降解的分子机制的结构洞察力。
影响因子：
16.6
作者：
Joo S;Cho IJ;Seo H;Son HF;Sagong HY;Shin TJ;Choi SY;Lee SY;Kim KJ
通讯作者：
Kim KJ