Divergence of cuticular hydrocarbons in two sympatric grasshopper species and the evolution of fatty acid synthases and elongases across insects.
Divergence of cuticular hydrocarbons in two sympatric grasshopper species and the evolution of fatty acid synthases and elongases across insects.
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DOI：
10.1038/srep33695
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发表时间：
2016-09-28
影响因子：
4.6
通讯作者：
Geiselhardt S
中科院分区：
文献类型：
--
作者：
Finck J;Berdan EL;Mayer F;Ronacher B;Geiselhardt S
表皮烃（Cuticular hydrocarbons, CHCs）在昆虫物种间生殖隔离的进化过程中起着重要作用。近缘同域蝗虫Chorthippus biguttulus和C. mollis的CHC谱差异主要体现在主要甲基支链CHC中第一甲基的位置。甲基分支的位置由脂肪酸合酶（FAS）或延长酶决定。这两个蛋白家族在昆虫中都有扩展。有趣的是，FAS家族显示出几个谱系特异性的扩展，特别是在具有高度多样化甲基支链CHC谱的昆虫目中。我们在两个物种的参考转录组中发现了5个推测的FASs和12个推测的延长酶。dN/dS测试显示，这些蚱蜢的FASs和延长酶没有正选择的证据。然而，一个候选FAS显示出物种特异性的转录差异，可能有助于物种之间甲基分支位置的转移。此外，四种伸长酶的转录水平在两性之间存在差异。我们的研究表明，复杂的甲基支链CHC谱与FASs基因的扩增有关，但物种差异也可以在转录水平上介导。
Cuticular hydrocarbons (CHCs) play a major role in the evolution of reproductive isolation between insect species. The CHC profiles of two closely related sympatric grasshopper species, Chorthippus biguttulus and C. mollis, differ mainly in the position of the first methyl group in major methyl-branched CHCs. The position of methyl branches is determined either by a fatty acid synthase (FAS) or by elongases. Both protein families showed an expansion in insects. Interestingly, the FAS family showed several lineage-specific expansions, especially in insect orders with highly diverse methyl-branched CHC profiles. We found five putative FASs and 12 putative elongases in the reference transcriptomes for both species. A dN/dS test showed no evidence for positive selection acting on FASs and elongases in these grasshoppers. However, one candidate FAS showed species-specific transcriptional differences and may contribute to the shift of the methyl-branch position between the species. In addition, transcript levels of four elongases were expressed differentially between the sexes. Our study indicates that complex methyl-branched CHC profiles are linked to an expansion of FASs genes, but that species differences can also mediated at the transcriptional level.
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影响因子：
8.8
作者：
Geiselhardt, Sven;Otte, Tobias;Hilker, Monika
通讯作者：
Hilker, Monika
影响因子：
14.9
作者：
Dönitz J;Schmitt-Engel C;Grossmann D;Gerischer L;Tech M;Schoppmeier M;Klingler M;Bucher G
通讯作者：
Bucher G
影响因子：
3.9
作者：
BUCKNER, JS;KOLATTUKUDY, PE;ROGERS, L
通讯作者：
ROGERS, L
DOI：
10.1073/pnas.0608142104
发表时间：
2007-03-13
影响因子：
11.1
作者：
Chertemps, Thomas;Duportets, Line;Wicker-Thomas, Claude
通讯作者：
Wicker-Thomas, Claude
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y