Improved tRNA prediction in the American house dust mite reveals widespread occurrence of extremely short minimal tRNAs in acariform mites.
Improved tRNA prediction in the American house dust mite reveals widespread occurrence of extremely short minimal tRNAs in acariform mites.
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DOI：
10.1186/1471-2164-10-598
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发表时间：
2009-12-11
期刊：
影响因子：
4.4
通讯作者：
Oconnor BM
中科院分区：
文献类型：
--
作者：
Klimov PB;Oconnor BM
非典型转运RNA是功能性最小的转运RNA，缺少D臂或T臂。它们比典型的三叶草转运RNA短得多。广泛存在的非典型的tRNA首先表现为secernentean线虫，后来在各种蜘蛛。越来越多的证据表明，某些螨类的tRNA甚至比线虫的最小tRNA还要短，这增加了缺乏D臂和T臂的tRNA可能存在于这些生物体中的可能性。在螨类中，特别是在屋尘螨属Dermatophagoides中，三叶草tRNA的存在仍然存在争议。粉尘螨的线粒体tRNA是最小的、非典型的tRNA，缺乏T臂或D臂。（49-62，54.4 ± 2.86 nt）显著高于对照组（p = 0.019）小于秀丽隐杆线虫（53-63，56.3 ± 2.30 nt），一个最小tRNA分类单元的模型。在Dermatophagoides中最短的tRNA（49 nt）接近已知最短的tRNA的长度（45-49 nt），在其他螨类中描述。在这些tRNA中不存在D臂，并且推断的T茎很小（2-3 bp）并且在分子上不稳定，这表明它可能在现实中不存在。在许多Dermatophagoides tRNAs中，nucleotide很可能不是编码的，而是转录后添加的。螨类的线粒体tRNA主要是非典型的，非苜蓿叶形的tRNA。其中，已知最短的tRNA没有D臂和短而不稳定的T臂可以推断。虽然我们的研究通过有限的EST数据证实了Dermatophagoides中的7种tRNA，但需要进一步的实验证据来证明螨类中非常小和不寻常的tRNA。
Atypical tRNAs are functional minimal tRNAs, lacking either the D- or T-arm. They are significantly shorter than typical cloverleaf tRNAs. Widespread occurrence of atypical tRNAs was first demonstrated for secernentean nematodes and later in various arachnids. Evidence started to accumulate that tRNAs of certain acariform mites are even shorter than the minimal tRNAs of nematodes, raising the possibility that tRNAs lacking both D- and T-arms might exist in these organisms. The presence of cloverleaf tRNAs in acariform mites, particularly in the house dust mite genus Dermatophagoides, is still disputed. Mitochondrial tRNAs of Dermatophagoides farinae are minimal, atypical tRNAs lacking either the T- or D-arm. The size (49-62, 54.4 ± 2.86 nt) is significantly (p = 0.019) smaller than in Caenorhabditis elegans (53-63, 56.3 ± 2.30 nt), a model minimal tRNA taxon. The shortest tRNA (49 nt) in Dermatophagoides is approaching the length of the shortest known tRNAs (45-49 nt) described in other acariform mites. The D-arm is absent in these tRNAs, and the inferred T-stem is small (2-3 bp) and thermodynamically unstable, suggesting that it may not exist in reality. The discriminator nucleotide is probably not encoded and is added postranscriptionally in many Dermatophagoides tRNAs. Mitochondrial tRNAs of acariform mites are largely atypical, non-cloverleaf tRNAs. Among them, the shortest known tRNAs with no D-arm and a short and unstable T-arm can be inferred. While our study confirmed seven tRNAs in Dermatophagoides by limited EST data, further experimental evidence is needed to demonstrate extremely small and unusual tRNAs in acariform mites.
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DOI：
10.1073/pnas.0400941101
发表时间：
2004-07-20
影响因子：
11.1
作者：
Helfenbein, KG;Fourcade, HM;Boore, JL
通讯作者：
Boore, JL
影响因子：
4.4
作者：
通讯作者：
--
影响因子：
14.9
作者：
Arita, Masashi;Suematsu, Takuma;Ohtsuki, Takashi
通讯作者：
Ohtsuki, Takashi
影响因子：
5.8
作者：
Laslett, Dean;Canback, Bjorn
通讯作者：
Canback, Bjorn
影响因子：
11.4
作者：
CLARKWALKER, GD;MCARTHUR, CR;SRIPRAKASH, KS
通讯作者：
SRIPRAKASH, KS