Odorant receptor expressed sequence tags demonstrate olfactory expression of over 400 genes, extensive alternate splicing and unequal expression levels.
Odorant receptor expressed sequence tags demonstrate olfactory expression of over 400 genes, extensive alternate splicing and unequal expression levels.
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DOI：
10.1186/gb-2003-4-11-r71
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发表时间：
2003
期刊：
影响因子：
12.3
通讯作者：
Trask BJ
中科院分区：
文献类型：
--
作者：
Young JM;Shykind BM;Lane RP;Tonnes-Priddy L;Ross JA;Walker M;Williams EM;Trask BJ
以前的计算分析已经确定了大约1,500个小鼠嗅觉受体，但实验证据证实嗅觉功能可用于极少数受体。筛选小鼠嗅上皮cDNA文库以获得嗅觉受体表达的序列标签，为许多其他嗅觉受体的嗅觉功能提供证据，以及鉴定基因结构和推定的启动子区域。嗅觉受体基因家族是哺乳动物基因组中最大的家族之一。以前的计算分析已经确定了大约1,500个小鼠嗅觉受体，但实验证据证实嗅觉功能可用于极少数嗅觉受体。因此，我们筛选了小鼠嗅上皮cDNA文库，以获得嗅觉受体表达的序列标签，为许多其他嗅觉受体的嗅觉功能提供证据，以及确定基因结构和推定的启动子区域。我们鉴定了1,200多个气味受体cDNA，代表了400多个基因。使用实时PCR来确认我们的筛选所建议的表达水平差异，我们发现嗅觉上皮中的转录水平在嗅觉受体之间可以相差高达300倍。一个基因对的差异显然是由于表达细胞的数量不相等和每个表达细胞的转录水平不相等。至少三分之二的嗅觉受体表现出多种转录变体，具有5'和3'非翻译区的替代同种型。一些转录本（5%）利用编码区内的剪接位点，与刻板的嗅觉受体基因结构相反。大多数非典型转录编码非功能性嗅觉受体，但偶尔可以增加受体的多样性。我们的cDNA收集证实了超过三分之一的完整小鼠嗅觉受体的嗅觉功能。这些基因中的大多数先前仅基于序列相似性被注释为嗅觉受体。我们的发现，不同的嗅觉受体有不同的表达水平是有趣的嗅觉受体的一个神经元，一个基因的表达制度。我们提供了超过300种嗅觉受体的5'非翻译区序列和候选启动子区域，为计算调控基序搜索和设计嗅觉受体微阵列和其他实验探针提供了宝贵的资源。
Previous computational analyses have identified approximately 1,500 mouse olfactory receptors, but experimental evidence confirming olfactory function is available for very few receptors. A mouse olfactory epithelium cDNA library was screened to obtain olfactory receptor expressed sequence tags, providing evidence of olfactory function for many additional olfactory receptors, as well as identifying gene structure and putative promoter regions. The olfactory receptor gene family is one of the largest in the mammalian genome. Previous computational analyses have identified approximately 1,500 mouse olfactory receptors, but experimental evidence confirming olfactory function is available for very few olfactory receptors. We therefore screened a mouse olfactory epithelium cDNA library to obtain olfactory receptor expressed sequence tags, providing evidence of olfactory function for many additional olfactory receptors, as well as identifying gene structure and putative promoter regions. We identified more than 1,200 odorant receptor cDNAs representing more than 400 genes. Using real-time PCR to confirm expression level differences suggested by our screen, we find that transcript levels in the olfactory epithelium can differ between olfactory receptors by up to 300-fold. Differences for one gene pair are apparently due to both unequal numbers of expressing cells and unequal transcript levels per expressing cell. At least two-thirds of olfactory receptors exhibit multiple transcriptional variants, with alternative isoforms of both 5' and 3' untranslated regions. Some transcripts (5%) utilize splice sites within the coding region, contrary to the stereotyped olfactory receptor gene structure. Most atypical transcripts encode nonfunctional olfactory receptors, but can occasionally increase receptor diversity. Our cDNA collection confirms olfactory function of over one-third of the intact mouse olfactory receptors. Most of these genes were previously annotated as olfactory receptors based solely on sequence similarity. Our finding that different olfactory receptors have different expression levels is intriguing given the one-neuron, one-gene expression regime of olfactory receptors. We provide 5' untranslated region sequences and candidate promoter regions for more than 300 olfactory receptors, valuable resources for computational regulatory motif searches and for designing olfactory receptor microarrays and other experimental probes.
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影响因子：
7
作者：
Kan, ZY;Rouchka, EC;States, DJ
通讯作者：
States, DJ
影响因子：
64.5
作者：
Malnic, B;Hirono, J;Buck, LB
通讯作者：
Buck, LB
DOI：
10.1073/pnas.94.9.4766
发表时间：
1997-04-29
影响因子：
11.1
作者：
Nef, S;Nef, P
通讯作者：
Nef, P
影响因子：
3.5
作者：
Linardopoulou, E;Mefford, HC;Trask, BJ
通讯作者：
Trask, BJ
DOI：
10.1073/pnas.97.26.14560
发表时间：
2000-12-19
影响因子：
11.1
作者：
Bulger, M;Bender, MA;Hardison, R
通讯作者：
Hardison, R