Expression study of the hunchback ortholog in embryos of the onychophoran Euperipatoides rowelli
Expression study of the hunchback ortholog in embryos of the onychophoran Euperipatoides rowelli
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驼背直系同源基因在甲虫Euperipatoides rowelli胚胎中的表达研究
DOI：
10.1007/s00427-015-0505-4
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发表时间：
2015
影响因子：
2.4
通讯作者：
Franke
中科院分区：
文献类型：
--
作者：
Franke
hunchbackhomologs编码的锌指转录因子在节肢动物中起着不同的作用，包括母系调控、分裂、中胚层和神经发育。在蜘蛛和昆虫胚胎中的敲除实验也揭示了同源异型效应和缺口表型，后者表明hunchback作为“缺口基因”的功能。虽然hunchback的表达模式已在所有四个主要的节肢动物群（螯肢类，多足类，甲壳类和昆虫）的代表进行了分析，没有什么是知道它的表达在一个最接近的节肢动物亲属，Onychophora（天鹅绒蠕虫）。因此，我们研究了hunchback在有爪类Euperipatoides rowelli胚胎中的表达模式。我们的转录组学和系统发育分析显示，只有一个hunchbackortholog在这个物种。推测的驼背蛋白含有其他原口动物已知的所有九个锌指结构域。我们在E. rowelli。其最初的表达发生在触角节的外胚层组织中，然后是颚、粘液乳头和躯干节中的前到后的进展。后来，hunchbackexpression被认为是在中胚层的发展肢体。第二个“波”的表达开始于触角节的发育后期，并沿沿着每个发育中的神经索向后继续。这种表达仅限于神经组织，不显示任何节段性模式。这些发现与hunchback在中胚层和神经发育中的祖先作用一致，而我们没有发现hunchback在甲属中作为“间隙基因”的假定功能的证据。
Zinc finger transcription factors encoded byhunchbackhomologs play different roles in arthropods, including maternally mediated control, segmentation, and mesoderm and neural development. Knockdown experiments in spider and insect embryos have also revealed homeotic effects and gap phenotypes, the latter indicating a function ofhunchbackas a “gap gene”. Although the expression pattern ofhunchbackhas been analysed in representatives of all four major arthropod groups (chelicerates, myriapods, crustaceans and insects), nothing is known about its expression in one of the closest arthropod relatives, the Onychophora (velvet worms). We therefore examined the expression pattern ofhunchbackin embryos of the onychophoranEuperipatoides rowelli. Our transcriptomic and phylogenetic analyses revealed only onehunchbackortholog in this species. The putative Hunchback protein contains all nine zinc finger domains known from other protostomes. We found no indication of maternally contributed transcripts ofhunchbackin early embryos ofE. rowelli. Its initial expression occurs in the ectodermal tissue of the antennal segment, followed by the jaw, slime papilla and trunk segments in an anterior-to-posterior progression. Later,hunchbackexpression is seen in the mesoderm of the developing limbs. A second “wave” of expression commences later in development in the antennal segment and continues posteriorly along each developing nerve cord. This expression is restricted to the neural tissues and does not show any segmental pattern. These findings are in line with the ancestral roles ofhunchbackin mesoderm and neural development, whereas we find no evidence for a putative function ofhunchbackas a “gap gene” in Onychophora.
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影响因子：
2.7
作者：
LEHMANN, R;NUSSLEINVOLHARD, C
通讯作者：
NUSSLEINVOLHARD, C
影响因子：
10.7
作者：
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通讯作者：
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影响因子：
9.2
作者：
Evelyn E. Schwager;Matthias Pechmann;N. M. Feitosa;A. McGregor;W. Damen
通讯作者：
W. Damen
影响因子：
6.8
作者：
AKAIKE, H
通讯作者：
AKAIKE, H
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影响因子：
4.6
作者：
M. Wilson;P. Dearden
通讯作者：
P. Dearden