Printing of Three-Dimensional Tissue Analogs for Regenerative Medicine.
Printing of Three-Dimensional Tissue Analogs for Regenerative Medicine.
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DOI：
10.1007/s10439-016-1613-7
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发表时间：
2017-01
影响因子：
3.8
通讯作者：
Dai G
中科院分区：
文献类型：
--
作者：
Lee VK;Dai G
3D细胞打印可以精确地将细胞、生物材料支架和生长因子存款在精确限定的空间模式中以形成仿生组织结构，已经成为创建用于药物发现和组织工程应用的活组织和器官结构的强大的使能技术。与使用金属、塑料和聚合物作为打印材料的传统3D打印不同，细胞打印必须与活细胞和生物基质兼容。还要求打印过程保留细胞和细胞外基质的生物学功能，并模仿天然组织的细胞-基质结构和机械性质。因此，要将传统的3D打印技术转化为细胞打印，并最终在打印的组织中实现功能性结果，存在重大挑战。因此，有必要开发专门用于细胞打印的新技术，并在生物打印组织中进行深入的基础研究，例如开发专门用于细胞打印应用的新型生物材料，了解复杂的细胞-基质重塑以获得所需的机械性能和功能结果，在生物打印组织中建立适当的血管灌注等。近年来，三维细胞打印技术及其在活体组织工程中的应用取得了令人振奋的研究进展。本文综述了3D细胞打印技术的发展现状，重点介绍了活体打印组织的结果及其在药物发现和再生医学中的潜在应用。强调了当前的挑战和限制，并讨论了3D细胞打印技术的未来发展方向。
3-D cell printing, which can accurately deposit cells, biomaterial scaffolds and growth factors in precisely defined spatial patterns to form biomimetic tissue structures, has emerged as a powerful enabling technology to create live tissue and organ structures for drug discovery and tissue engineering applications. Unlike traditional 3-D printing that uses metals, plastics and polymers as the printing materials, cell printing has to be compatible with living cells and biological matrix. It is also required that the printing process preserves the biological functions of the cells and extracellular matrix, and to mimic the cell-matrix architectures and mechanical properties of the native tissues. Therefore, there are significant challenges in order to translate the technologies of traditional 3-D printing to cell printing, and ultimately achieve functional outcomes in the printed tissues. So it is essential to develop new technologies specially designed for cell printing and in-depth basic research in the bioprinted tissues, such as developing novel biomaterials specifically for cell printing applications, understanding the complex cell-matrix remodeling for the desired mechanical properties and functional outcomes, establishing proper vascular perfusion in bioprinted tissues, etc. In recent years, many exciting research progresses have been made in the 3-D cell printing technology and its application in engineering live tissue constructs. This review paper summarized the current development in 3-D cell printing technologies; focus on the outcomes of the live printed tissues and their potential applications in drug discovery and regenerative medicine. Current challenges and limitations are highlighted, and future directions of 3-D cell printing technology are also discussed.
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影响因子：
4.1
作者：
Ballyns, Jeffery J.;Gleghorn, Jason P.;Bonassar, Lawrence J.
通讯作者：
Bonassar, Lawrence J.
影响因子：
1.2
作者：
Cui, Xiaofeng;Gao, Guifang;Dai, Guohao
通讯作者：
Dai, Guohao
影响因子：
14
作者：
Gao, Qing;He, Yong;Ma, Liang
通讯作者：
Ma, Liang
影响因子：
3.6
作者：
Giordano, RA;Wu, BM;Cima, MJ
通讯作者：
Cima, MJ
影响因子：
14
作者：
Gaetani, Roberto;Feyen, Dries A. M.;Sluijter, Joost P. G.
通讯作者：
Sluijter, Joost P. G.