Characterization of the cold-sensitive murine hepatitis virus mutants rescued from latently infected cells by cell fusion.
Characterization of the cold-sensitive murine hepatitis virus mutants rescued from latently infected cells by cell fusion.
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DOI：
10.1016/0042-6822(79)90567-1
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发表时间：
1979-10-30
期刊：
影响因子：
3.7
通讯作者：
Weiner LP
中科院分区：
文献类型：
--
作者：
Stohlman SA;Sakaguchi AY;Weiner LP
小鼠肝炎病毒，一种冠状病毒，从潜伏感染的小鼠神经母细胞瘤细胞中被聚乙二醇诱导融合到允许细胞中。分离的病毒在39°下生长至等于或高于亲本JHM病毒的滴度，但在32°下复制受限，因此是冷敏感突变体。两个分离株在非允许温度（32°）下都不能合成RNA，温度变化实验表明，复制中的限制性冷敏感步骤发生在早期病毒功能期间。分离株在32°下不能相互补充。虽然每个突变体和亲本JHM病毒之间的共感染往往会导致两种感染病毒的产量下降，干扰不能令人信服地证明。这两种突变体比亲本JHM病毒更耐热，并且可以通过它们的热灭活动力学相互区分以及与亲本JHM病毒区分。从单细胞克隆中获得分离株，因此推测为同质;然而，一个（S-3 JHM）的热灭活表明可能存在第二个群体。还发现从每个分离物制备的病毒亚克隆是热稳定的，并保留冷敏感缺陷。未发现S-3 JHM病毒第二个种群的迹象。
Mouse hepatitis virus, a coronavirus, was rescued from latently infected mouse neuroblastoma cells by polyethylene glycol-induced fusion to permissive cells. The isolated viruses grew to equal or higher titers than parental JHM virus at 39° but were restricted in replication at 32° and hence were cold-sensitive mutants. Neither isolate synthesized RNA at the nonpermissive temperature (32°), and temperature shift experiments indicated that the restrictive cold-sensitive step in replication occurred during an early viral function. The isolates were unable to complement each other at 32°. Although coinfection between each mutant and parental JHM virus often resulted in decreased yields of both infecting viruses, interference could not be convincingly demonstrated. Both mutants were more thermostable than parental JHM virus and could be distinguished from each other and from parental JHM virus by their thermal inactivation kinetics. The isolates were obtained from single cell clones and therefore presumed to be homogeneous; however, thermal inactivation of one (S-3JHM) indicated the possible existence of a second population. Subclones of virus prepared from each isolate were also found to be thermally stable and to retain the cold sensitive defect. No indication of a second population of S-3JHM virus was detected.
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DOI：
10.1073/pnas.70.7.2046
发表时间：
1973-01-01
影响因子：
11.1
作者：
JARVIK, J;BOTSTEIN, D
通讯作者：
BOTSTEIN, D
影响因子：
--
作者：
WEINER, LP
通讯作者：
WEINER, LP
影响因子：
6.1
作者：
STOHLMAN, SA;SAKAGUCHI, AY;WEINER, LP
通讯作者：
WEINER, LP
影响因子：
4.6
作者：
WRIGHT, PJ;COOPER, PD
通讯作者：
COOPER, PD
影响因子：
5.4
作者：
LAI, MMC;STOHLMAN, SA
通讯作者：
STOHLMAN, SA