Ciliary neurotrophic factor promotes motor reinnervation of the musculocutaneous nerve in an experimental model of end-to-side neurorrhaphy.
Ciliary neurotrophic factor promotes motor reinnervation of the musculocutaneous nerve in an experimental model of end-to-side neurorrhaphy.
复制标题
DOI：
10.1186/1471-2202-12-58
复制
发表时间：
2011-06-22
期刊：
影响因子：
2.4
通讯作者：
Haninec P
中科院分区：
文献类型：
--
作者：
Dubový P;Raška O;Klusáková I;Stejskal L;Celakovský P;Haninec P
如果近端残端缺失或自体神经移植物不足以重建大面积神经损伤，神经修复是困难的。因此，备选的方法被开发出来，包括基于轴突向失神经发送侧支芽的能力的侧侧吻合。感觉轴突和运动轴突发送芽的不同能力是有争议的，可能受细胞因子和/或神经营养因子如睫状体神经营养因子（CNTF）的控制。本研究的目的是定量评估在端侧神经吻合模型中，鞘内给药CNTF后，由完整的运动和感觉轴突发出的侧支芽，并与磷酸缓冲盐水（载液）和脑溶素进行比较。将大鼠远端断肌皮神经端侧与尺神经连接。经鞘内给予CNTF、脑溶血素和载药2周，术后所有动物均存活2个月。用荧光红宝石和荧光祖母绿分别逆行标记尺神经和肌皮神经后，估计脊髓运动神经元和背根神经节神经元的数量。采用肌电图、行为学测试、再生轴突直径和髓鞘厚度评价肱二头肌神经再生情况。与CNTF治疗相比，给药或溶脑素导致MCN残端再生的髓鞘轴突数量显著增加。相比之下，脑溶素或cntf处理的动物有髓轴突的平均直径和髓鞘厚度比用运载物处理的大鼠大。与运载物或溶脑素治疗（分别为9.9或9.6%）相比，CNTF治疗显著增加了有助于MCN残端再神经支配的运动神经元百分比（17.1%）。CNTF治疗后，髓鞘轴突数量减少，同时运动神经元数量增加，有助于MCN的再神经支配，改善了肱二头肌的功能性再神经支配。目前的实验研究证实神经端侧吻合是一种重建周围神经的替代方法。CNTF促进MCN残端与尺神经端侧吻合后的运动神经再生，改善肱二头肌功能恢复。
It is difficult to repair nerve if proximal stump is unavailable or autogenous nerve grafts are insufficient for reconstructing extensive nerve damage. Therefore, alternative methods have been developed, including lateral anastomosis based on axons' ability to send out collateral sprouts into denervated nerve. The different capacity of a sensory or motor axon to send a sprout is controversial and may be controlled by cytokines and/or neurotrophic factors like ciliary neurotrophic factor (CNTF). The aim of the present study was to quantitatively assess collateral sprouts sent out by intact motor and sensory axons in the end-to-side neurorrhaphy model following intrathecal administration of CNTF in comparison with phosphate buffered saline (vehiculum) and Cerebrolysin. The distal stump of rat transected musculocutaneous nerve (MCN) was attached in an end-to-side fashion with ulnar nerve. CNTF, Cerebrolysin and vehiculum were administered intrathecally for 2 weeks, and all animals were allowed to survive for 2 months from operation. Numbers of spinal motor and dorsal root ganglia neurons were estimated following their retrograde labeling by Fluoro-Ruby and Fluoro-Emerald applied to ulnar and musculocutaneous nerve, respectively. Reinnervation of biceps brachii muscles was assessed by electromyography, behavioral test, and diameter and myelin sheath thickness of regenerated axons. Vehiculum or Cerebrolysin administration resulted in significantly higher numbers of myelinated axons regenerated into the MCN stumps compared with CNTF treatment. By contrast, the mean diameter of the myelinated axons and their myelin sheath thickness in the cases of Cerebrolysin- or CNTF-treated animals were larger than were those for rats treated with vehiculum. CNTF treatment significantly increased the percentage of motoneurons contributing to reinnervation of the MCN stumps (to 17.1%) when compared with vehiculum or Cerebrolysin treatments (at 9.9 or 9.6%, respectively). Reduced numbers of myelinated axons and simultaneously increased numbers of motoneurons contributing to reinnervation of the MCN improved functional reinnervation of the biceps brachii muscle after CNTF treatment. The present experimental study confirms end-to-side neurorrhaphy as an alternative method for reconstructing severed peripheral nerves. CNTF promotes motor reinnervation of the MCN stump after its end-to-side neurorrhaphy with ulnar nerve and improves functional recovery of the biceps brachii muscle.
登录
查看更多内容
影响因子：
2
作者：
Haninec, P;Dubovy, P;Stejskal, L
通讯作者：
Stejskal, L
影响因子：
4.1
作者：
Haninec, Pavel;Samal, Filip;Dubovy, Petr
通讯作者：
Dubovy, Petr
影响因子：
2.2
作者：
Haninec, P;Houst'ava, L;Dubovy, P
通讯作者：
Dubovy, P
DOI：
10.1016/0955-2235(92)90028-g
发表时间：
1992-01-01
期刊：
Progress in Growth Factor Research
影响因子：
--
作者：
Ip, Nancy Y.;Yancopoulos, George D.
通讯作者：
Yancopoulos, George D.
影响因子：
7.8
作者：
CARONI, P;GRANDES, P
通讯作者：
GRANDES, P