Auxin regulated metabolic changes underlying sepal retention and development after pollination in spinach.
Auxin regulated metabolic changes underlying sepal retention and development after pollination in spinach.
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DOI：
10.1186/s12870-021-02944-4
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发表时间：
2021-04-06
影响因子：
5.3
通讯作者：
Ming R
中科院分区：
文献类型：
--
作者：
Fatima M;Ma X;Zhou P;Zaynab M;Ming R
花粉囊通过保护雌性菠菜的种子来加速萼片的发育，从而增强植物的适应性。这种反应需要授粉信号，导致萼片细胞内的重塑，以保持和发展，但这种反应的调控机制仍然不清楚。为了研究早期授粉诱导的萼片代谢变化，我们利用高通量RNA-seq方法。以菠菜品种‘Cornel 9’为材料，进行了生长素类似物和生长素抑制剂处理的差异表达基因分析。我们首先比较了授粉后不同时间点（12 H、48 H和96 H）差异表达的候选转录本，发现它们在依赖色氨酸的生长素生物合成和生长素调控及转导过程中存在显著差异。此外，几个生长素调节途径，即细胞分裂，细胞壁扩张，和生物发生激活授粉后萼片的早期发育症状。为了进一步证实生长素基因在萼片发育中的作用，分别用不同浓度梯度的生长素类似物（2，4-D; IAA）和生长素转运抑制剂（NPA）对菠菜未授粉和授粉花进行喷雾处理。NPA处理导致生长素运输减弱，导致授粉后浓度为0.1和1 mM的萼片发育的抑制。2，4-D和IAA处理未授粉的花，在低浓度下导致萼片发育，但在高浓度下导致萎蔫。我们推测，萼片的保留和发展可能与生长素的动态平衡，调节相关的途径调节萼片的大小。这些发现促进了对菠菜授粉后萼片生长而不是脱落这一不寻常现象的理解。在线版本包含补充材料，可通过10.1186/s12870-021-02944-4获得。
Pollination accelerate sepal development that enhances plant fitness by protecting seeds in female spinach. This response requires pollination signals that result in the remodeling within the sepal cells for retention and development, but the regulatory mechanism for this response is still unclear. To investigate the early pollination-induced metabolic changes in sepal, we utilize the high-throughput RNA-seq approach. Spinach variety ‘Cornel 9’ was used for differentially expressed gene analysis followed by experiments of auxin analog and auxin inhibitor treatments. We first compared the candidate transcripts expressed differentially at different time points (12H, 48H, and 96H) after pollination and detected significant difference in Trp-dependent auxin biosynthesis and auxin modulation and transduction process. Furthermore, several auxin regulatory pathways i.e. cell division, cell wall expansion, and biogenesis were activated from pollination to early developmental symptoms in sepals following pollination. To further confirm the role auxin genes play in the sepal development, auxin analog (2, 4-D; IAA) and auxin transport inhibitor (NPA) with different concentrations gradient were sprayed to the spinach unpollinated and pollinated flowers, respectively. NPA treatment resulted in auxin transport weakening that led to inhibition of sepal development at concentration 0.1 and 1 mM after pollination. 2, 4-D and IAA treatment to unpollinated flowers resulted in sepal development at lower concentration but wilting at higher concentration. We hypothesized that sepal retention and development might have associated with auxin homeostasis that regulates the sepal size by modulating associated pathways. These findings advanced the understanding of this unusual phenomenon of sepal growth instead of abscission after pollination in spinach. The online version contains supplementary material available at 10.1186/s12870-021-02944-4.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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