Scanning laser optical tomography resolves structural plasticity during regeneration in an insect brain.
Scanning laser optical tomography resolves structural plasticity during regeneration in an insect brain.
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DOI：
10.1371/journal.pone.0041236
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Bicker G
中科院分区：
文献类型：
--
作者：
Eickhoff R;Lorbeer RA;Scheiblich H;Heisterkamp A;Meyer H;Stern M;Bicker G
光学投影层析成像（OPT）是一种显微技术，其从尺寸超过共焦激光扫描显微镜的最大焦深的整个安装样品产生三维图像。扫描激光光学层析成像（SLOTy）是传统发射-光学层析成像的一个进步，它可以同时检测荧光和吸光度，具有高灵敏度。在本研究中，我们采用SLOTy在昆虫模型系统的大脑可塑性的范例。我们可视化和量化的体积变化的感觉信息处理中心的成年蝗虫，飞蝗。嗅觉受体神经元从触角投射到大脑，通过压碎触角神经或消融整个触角来切断。我们通过在重建的SLOTy图像中测量它们相对于未处理的对照侧的大小来跟踪嗅觉中心（触角叶和蘑菇体）中的退化和再生。治疗后三周内，触角被烧蚀的触角叶损失了初始体积的60%。相反，触角神经被压碎的触角叶最初也会缩小，但在三周内恢复正常。神经生物素标记的轴突切断的纤维的透射和荧光投影的组合应用证实了正常大小的恢复是由再生的传入恢复。值得注意的是，SLOTy图像揭示了嗅觉受体轴突的变性对二级脑中心具有跨突触效应，并导致蘑菇体萼的尺寸减小。本研究表明，SLOTy是一个合适的方法，用于快速筛选昆虫脑的体积可塑性，并建议其应用也脊椎动物制剂。
Optical Projection Tomography (OPT) is a microscopic technique that generates three dimensional images from whole mount samples the size of which exceeds the maximum focal depth of confocal laser scanning microscopes. As an advancement of conventional emission-OPT, Scanning Laser Optical Tomography (SLOTy) allows simultaneous detection of fluorescence and absorbance with high sensitivity. In the present study, we employ SLOTy in a paradigm of brain plasticity in an insect model system. We visualize and quantify volumetric changes in sensory information procession centers in the adult locust, Locusta migratoria. Olfactory receptor neurons, which project from the antenna into the brain, are axotomized by crushing the antennal nerve or ablating the entire antenna. We follow the resulting degeneration and regeneration in the olfactory centers (antennal lobes and mushroom bodies) by measuring their size in reconstructed SLOTy images with respect to the untreated control side. Within three weeks post treatment antennal lobes with ablated antennae lose as much as 60% of their initial volume. In contrast, antennal lobes with crushed antennal nerves initially shrink as well, but regain size back to normal within three weeks. The combined application of transmission-and fluorescence projections of Neurobiotin labeled axotomized fibers confirms that recovery of normal size is restored by regenerated afferents. Remarkably, SLOTy images reveal that degeneration of olfactory receptor axons has a trans-synaptic effect on second order brain centers and leads to size reduction of the mushroom body calyx. This study demonstrates that SLOTy is a suitable method for rapid screening of volumetric plasticity in insect brains and suggests its application also to vertebrate preparations.
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DOI：
10.1016/j.devbrainres.2004.02.006
发表时间：
2004-05-19
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
Pätschke, A;Bicker, G;Stern, M
通讯作者：
Stern, M
影响因子：
5.3
作者：
HEISENBERG, M;HEUSIPP, M;WANKE, C
通讯作者：
WANKE, C
影响因子：
11.6
作者：
Lee, Karen;Avondo, Jerome;Coen, Enrico
通讯作者：
Coen, Enrico
影响因子：
2.6
作者：
HOUNDFIELD, GN
通讯作者：
HOUNDFIELD, GN
影响因子：
5.3
作者：
Ayaz, Derya;Leyssen, Maarten;Hassan, Bassem A.
通讯作者：
Hassan, Bassem A.