The Imd pathway is involved in antiviral immune responses in Drosophila.
The Imd pathway is involved in antiviral immune responses in Drosophila.
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DOI：
10.1371/journal.pone.0007436
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发表时间：
2009-10-15
期刊：
影响因子：
3.7
通讯作者：
Schneider D
中科院分区：
文献类型：
--
作者：
Costa A;Jan E;Sarnow P;Schneider D
蟋蟀麻痹病毒（CrPV）是双链病毒科RNA病毒的成员，其感染广泛的昆虫宿主，包括果蝇（Drosophila melanogaster）。果蝇因其强大的遗传技术和高度的基因和途径保守性而成为研究先天免疫的有效系统。将CrPV腹腔内注射到成年苍蝇中会引起致死性感染，这为鉴定对病毒感染敏感性改变的突变体提供了一种稳健的检测方法。为了深入了解病毒和先天免疫系统之间的相互作用，我们用CrPV注射野生型果蝇，观察到在感染过程中没有诱导抗菌肽（AMP），血细胞被耗尽。为了研究保守的免疫信号传导途径对抗病毒先天免疫应答的贡献，将CrPV注射到免疫缺陷（Imd）途径的同基因突变体中，所述免疫缺陷（Imd）途径类似于哺乳动物肿瘤坏死因子受体（TNFR）途径。几个Imd途径基因的功能丧失突变显示出对CrPV感染的敏感性增加和CrPV负荷增加。我们的数据表明，感染CrPV的果蝇的抗病毒先天免疫应答依赖于血细胞和通过Imd途径的信号传导。
Cricket Paralysis virus (CrPV) is a member of the Dicistroviridae family of RNA viruses, which infect a broad range of insect hosts, including the fruit fly Drosophila melanogaster. Drosophila has emerged as an effective system for studying innate immunity because of its powerful genetic techniques and the high degree of gene and pathway conservation. Intra-abdominal injection of CrPV into adult flies causes a lethal infection that provides a robust assay for the identification of mutants with altered sensitivity to viral infection. To gain insight into the interactions between viruses and the innate immune system, we injected wild type flies with CrPV and observed that antimicrobial peptides (AMPs) were not induced and hemocytes were depleted in the course of infection. To investigate the contribution of conserved immune signaling pathways to antiviral innate immune responses, CrPV was injected into isogenic mutants of the Immune Deficiency (Imd) pathway, which resembles the mammalian Tumor Necrosis Factor Receptor (TNFR) pathway. Loss-of-function mutations in several Imd pathway genes displayed increased sensitivity to CrPV infection and higher CrPV loads. Our data show that antiviral innate immune responses in flies infected with CrPV depend upon hemocytes and signaling through the Imd pathway.
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影响因子：
3.3
作者：
Ayres, Janelle S.;Freitag, Nancy;Schneider, David S.
通讯作者：
Schneider, David S.
DOI：
10.1073/pnas.0404952102
发表时间：
2005-01-25
影响因子：
11.1
作者：
Choe, KM;Lee, H;Anderson, KV
通讯作者：
Anderson, KV
影响因子：
4.8
作者：
Hedengren-Olcott, M;Olcott, MC;Taylor, BJ
通讯作者：
Taylor, BJ
影响因子：
2.1
作者：
Corby-Harris, V.;Habel, K. E.;Promislow, D. E. L.
通讯作者：
Promislow, D. E. L.
影响因子：
3.8
作者：
Hedges, Lauren M.;Johnson, Karyn N.
通讯作者：
Johnson, Karyn N.