Redox-sensitive probes for the measurement of redox chemistries within phagosomes of macrophages and dendritic cells.
Redox-sensitive probes for the measurement of redox chemistries within phagosomes of macrophages and dendritic cells.
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DOI：
10.1016/j.redox.2013.09.002
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发表时间：
2013-09-27
期刊：
影响因子：
11.4
通讯作者：
Yates, Robin M.
中科院分区：
文献类型：
--
作者：
Balce, Dale R.;Yates, Robin M.
目前人们对影响巨噬细胞和树突状细胞吞噬体内氧化还原化学的因素很感兴趣。除了吞噬体内产生的活性氧物质的抗菌作用外，越来越多的证据表明，吞噬体氧化还原化学作用还影响其他吞噬体功能，如大分子降解和抗原处理。虽然随着荧光探针技术的使用越来越多，许多亚细胞内的氧化还原化学物质受到了严格的审查，但评估吞噬小体内的氧化还原条件的工具很少。因此，在这些独特的环境中控制氧化还原动态平衡的系统仍然没有得到很好的定义。本文重点介绍了目前可以测量吞噬体内氧化或还原活性的氧化还原敏感探针，并讨论了它们的适用性和使用限制。重点介绍了利用已建立的方法容易地以噬菌体为靶标的探针。氧化还原探针及其在巨噬细胞和树突状细胞吞噬小体中的应用强调了允许吞噬体特异性氧化还原测量的技术。包括了最常用的氧化还原探头的优点和注意事项。
There is currently much interest in factors that affect redox chemistries within phagosomes of macrophages and dendritic cells. In addition to the antimicrobial role of reactive oxygen species generation within phagosomes, accumulating evidence suggests that phagosomal redox chemistries influence other phagosomal functions such as macromolecular degradation and antigen processing. Whilst the redox chemistries within many sub-cellular compartments are being heavily scrutinized with the increasing use of fluorescent probe technologies, there is a paucity of tools to assess redox conditions within phagosomes. Hence the systems that control redox homeostasis in these unique environments remain poorly defined. This review highlights current redox-sensitive probes that can measure oxidative or reductive activity in phagosomes and discusses their suitability and limitations of use. Probes that are easily targeted to the phagosome by using established approaches are emphasized. A review of redox probes and their use in macrophage and dendritic cell phagosomes. Techniques that allow for phagosomal-specific redox measurements are highlighted. Advantages and caveats of the most commonly used redox probes are included.
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DOI：
10.1073/pnas.97.2.745
发表时间：
2000-01-18
影响因子：
11.1
作者：
Arunachalam, B;Phan, UT;Cresswell, P
通讯作者：
Cresswell, P
影响因子：
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DOI：
10.1016/0006-291x(72)90545-1
发表时间：
1972-01-01
影响因子：
3.1
作者：
ALLEN, RC;STEELE, RH;STJERNHOLM, RL
通讯作者：
STJERNHOLM, RL
DOI：
10.1073/pnas.0509035102
发表时间：
2005-12-13
影响因子：
11.1
作者：
Austin, CD;Wen, XH;Scales, SJ
通讯作者：
Scales, SJ
佛波酯可诱导一部分溶酶体氧化活性升高和碱化。
影响因子：
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作者：
Chen CS
通讯作者：
Chen CS