Time-lapse fluorescence imaging and quantitative single cell and endosomal analysis of peritoneal macrophages using fluorescent organosilica nanoparticles.
Time-lapse fluorescence imaging and quantitative single cell and endosomal analysis of peritoneal macrophages using fluorescent organosilica nanoparticles.
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使用荧光有机二氧化硅纳米粒子对腹膜巨噬细胞进行延时荧光成像和定量单细胞和内体分析。
DOI：
10.1016/j.nano.2012.05.018
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
K. Ishimura
中科院分区：
文献类型：
--
作者：
Michihiro Nakamura;K. Miyamoto;Koichiro Hayashi;A. Awaad;M. Ochiai;K. Ishimura
将具有100 nm直径的荧光硫醇-有机二氧化硅纳米颗粒（F-thiol-OS-100）应用于延时荧光成像。与量子点和有机荧光染料相比，F-thiol-OS-100在定量分析方面具有很大的优势。使用F-thiol-OS-100的小鼠腹腔巨噬细胞的延时荧光成像清楚地证明了细胞摄取，并且单细胞分析显示了可以定量评价的各种摄取动力学模式。我们还进行了单细胞内体摄取和运动的定量分析。还观察并定量分析了形态学结果与内体摄取和运动随时间的相关性。F-thiol-OS-100作为一种新的荧光标记物显示出很高的潜力，用于延时荧光成像和定量单细胞功能分析，用于纳米医学开发。来自临床编辑：在这项研究中，作者报告了100 nm硫醇-有机硅纳米颗粒作为延时荧光标记物。F-thiol-OS-100被证明是优于量子点和有机荧光染料的上级荧光染料，并且能够进行定量单细胞功能分析。
Fluorescent thiol-organosilica nanoparticles with 100 nm diameter (F-thiol-OS-100) were applied for time-lapse fluorescence imaging. The evaluation of F-thiol-OS-100 for quantitative analysis demonstrated great advantages as compared with quantum dots and organic fluorescent dye. Time-lapse fluorescence imaging of mouse peritoneal macrophages using F-thiol-OS-100 clearly demonstrated cellular uptake, and single cell analysis showed various patterns of uptake kinetics that could be quantitatively evaluated. We also performed quantitative analysis of endosomal uptake and movements in single cells. A correlation between morphologic findings and endosomal uptake and movement over time was also observed and analyzed quantitatively. The F-thiol-OS-100 showed high potential as a new fluorescence marker for time-lapse fluorescence imaging and quantitative single cell functional analysis for nanomedicine development. FROM THE CLINICAL EDITOR: In this study the authors report on 100 nm thiol-organosilica nanoparticles as time-lapse flurescent markers. F-thiol-OS-100 proved to be superior to quantum dots and organic flurescent dyes, and enabled quantitative single cell functional analysis.
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影响因子：
4.9
作者：
Gullotti E;Yeo Y
通讯作者：
Yeo Y
影响因子：
10.8
作者：
Hulchanskyy, Tyrnish Y.;Roy, Indrajit;Prasad, Paras N.
通讯作者：
Prasad, Paras N.
影响因子：
4.4
作者：
Durazo, Shelley A.;Kompella, Uday B.
通讯作者：
Kompella, Uday B.
使用鲎顶体过程和基于光镊的测定法进行免疫吸附脑肌球蛋白-V 的体外运动。
影响因子：
4
作者：
Wolenski,JS;Cheney,RE;Mooseker,MS;Forscher,P
通讯作者：
Forscher,P
DOI：
10.1016/j.jconrel.2010.03.016
发表时间：
2010-08-03
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Li SD;Huang L
通讯作者：
Huang L