OsJAZ11 regulates phosphate starvation responses in rice.
OsJAZ11 regulates phosphate starvation responses in rice.
复制标题
DOI：
10.1007/s00425-021-03657-6
复制
发表时间：
2021-06-18
期刊：
影响因子：
4.3
通讯作者：
Mehra P
中科院分区：
文献类型：
--
作者：
Pandey BK;Verma L;Prusty A;Singh AP;Bennett MJ;Tyagi AK;Giri J;Mehra P
OsJAZ11通过抑制茉莉酸信号转导和生物合成来调节水稻根中的磷酸盐动态平衡。茉莉酸(JA)是一种关键的植物信号分子，对包括根伸长在内的生长过程起着负性调节作用。JAZ(茉莉酸ZIM结构域)蛋白是JA信号的转录抑制因子。因此，通过部署Jaz抑制因子来靶向JA信号转导可能会增加作物的根长。在本研究中，我们在水稻中过表达JAZ抑制基因OsJAZ11，以缓解JA对根生长的抑制作用。OsJAZ11是一个受OsPHR2转录调控的低磷反应基因。我们报告了OsJAZ11的过表达促进了初生根和种子根的伸长，从而促进了PI的觅食。表达研究表明，过表达OsJAZ11还降低了PI限制条件下的PI饥饿反应(PSR)。此外，与野生型(WT)相比，OsJAZ11过表达还抑制了JA信号和生物合成。我们进一步证明了OsJAZ11的C-末端区域对于刺激过表达品系的根伸长是至关重要的。过量表达截短的OsJAZ11ΔC基因(即缺失C-末端区域)的转基因水稻表现出根长和PI吸收减少。有趣的是，OsJAZ11还通过与关键的PI传感蛋白OsSPX1的物理相互作用来调节PI的动态平衡。我们的研究强调了JA和PI信号之间的功能联系，并揭示了JAZ抑制基因是一个有希望的候选基因，可以用来改善优良水稻品种的低PI耐受性。网上版载有补充材料，可在10.1007/s00425-021-03657-6查阅。
OsJAZ11 regulates phosphate homeostasis by suppressing jasmonic acid signaling and biosynthesis in rice roots. Jasmonic Acid (JA) is a key plant signaling molecule which negatively regulates growth processes including root elongation. JAZ (JASMONATE ZIM-DOMAIN) proteins function as transcriptional repressors of JA signaling. Therefore, targeting JA signaling by deploying JAZ repressors may enhance root length in crops. In this study, we overexpressed JAZ repressor OsJAZ11 in rice to alleviate the root growth inhibitory action of JA. OsJAZ11 is a low phosphate (Pi) responsive gene which is transcriptionally regulated by OsPHR2. We report that OsJAZ11 overexpression promoted primary and seminal root elongation which enhanced Pi foraging. Expression studies revealed that overexpression of OsJAZ11 also reduced Pi starvation response (PSR) under Pi limiting conditions. Moreover, OsJAZ11 overexpression also suppressed JA signaling and biosynthesis as compared to wild type (WT). We further demonstrated that the C-terminal region of OsJAZ11 was crucial for stimulating root elongation in overexpression lines. Rice transgenics overexpressing truncated OsJAZ11ΔC transgene (i.e., missing C-terminal region) exhibited reduced root length and Pi uptake. Interestingly, OsJAZ11 also regulates Pi homeostasis via physical interaction with a key Pi sensing protein, OsSPX1. Our study highlights the functional connections between JA and Pi signaling and reveals JAZ repressors as a promising candidate for improving low Pi tolerance of elite rice genotypes. The online version contains supplementary material available at 10.1007/s00425-021-03657-6.
登录
查看更多内容
影响因子：
4.6
作者：
Hsieh PH;Kan CC;Wu HY;Yang HC;Hsieh MH
通讯作者：
Hsieh MH
影响因子：
16.6
作者：
Bhosale R;Giri J;Pandey BK;Giehl RFH;Hartmann A;Traini R;Truskina J;Leftley N;Hanlon M;Swarup K;Rashed A;Voß U;Alonso J;Stepanova A;Yun J;Ljung K;Brown KM;Lynch JP;Dolan L;Vernoux T;Bishopp A;Wells D;von Wirén N;Bennett MJ;Swarup R
通讯作者：
Swarup R
影响因子：
16.6
作者：
Giri J;Bhosale R;Huang G;Pandey BK;Parker H;Zappala S;Yang J;Dievart A;Bureau C;Ljung K;Price A;Rose T;Larrieu A;Mairhofer S;Sturrock CJ;White P;Dupuy L;Hawkesford M;Perin C;Liang W;Peret B;Hodgman CT;Lynch J;Wissuwa M;Zhang D;Pridmore T;Mooney SJ;Guiderdoni E;Swarup R;Bennett MJ
通讯作者：
Bennett MJ
影响因子：
5.1
作者：
Kobayashi T;Itai RN;Senoura T;Oikawa T;Ishimaru Y;Ueda M;Nakanishi H;Nishizawa NK
通讯作者：
Nishizawa NK
影响因子：
2.3
作者：
Campos, Marcelo L.;Kang, Jin-Ho;Howe, Gregg A.
通讯作者：
Howe, Gregg A.