In Vivo Imaging of Nipah Virus Infection in Small Animal Rodent Models.
In Vivo Imaging of Nipah Virus Infection in Small Animal Rodent Models.
复制标题
小动物啮齿动物模型中尼帕病毒感染的体内成像。
DOI:
10.1007/978-1-0716-3283-3_11
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发表时间:
2023
期刊:
影响因子:
--
通讯作者:
Freiberg,AlexanderN
中科院分区:
文献类型:
--
作者:
Johnson,Kendra;Juelich,Terry;Smith,Jennifer;Lee,Benhur;Freiberg,AlexanderN
In vivo imaging system (IVIS) is a powerful tool for the study of infectious diseases, providing the ability to non-invasively follow viral infection in an individual animal over time. Recombinant henipaviruses expressing bioluminescent or fluorescent reporter proteins can be used both to monitor the spatial and temporal progression of Nipah virus (NiV) infection in vivo as well as in ex vivo tissues. Virally produced luciferases react with systemically administered substrate to produce bioluminescence that can then be detected via IVIS imaging, while fluorescent reporters inherently generate detectable fluorescence without a substrate. Here we describe protocols applying bioluminescent or fluorescent reporter expressing recombinant viruses to in vivo or ex vivo imaging of NiV infection.
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影响因子:
4.8
作者:
T. Cicero;B. Schainker;E. Meyer
通讯作者:
E. Meyer
影响因子:
8.3
作者:
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DOI:
--
发表时间:
1982
期刊:
影响因子:
--
作者:
R. Macholz
通讯作者:
R. Macholz
DOI:
10.1172/jci105937
发表时间:
1968
期刊:
The Journal of clinical investigation
影响因子:
--
作者:
W. Odell;A. Parlow;C. Cargille;G. Ross
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影响因子:
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通讯作者:
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