A fast one-step extraction and UPLC-MS/MS analysis for E2/D 2 series prostaglandins and isoprostanes.
A fast one-step extraction and UPLC-MS/MS analysis for E2/D 2 series prostaglandins and isoprostanes.
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DOI：
10.1007/s11745-013-3767-5
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发表时间：
2013-04
期刊：
影响因子：
1.9
通讯作者：
Golovko MY
中科院分区：
文献类型：
--
作者：
Brose SA;Baker AG;Golovko MY
前列腺素(PG)和异前列腺素(iso-PG)可通过环氧合酶或自由基途径产生，是重要的信号分子，也是氧化应激的强大生物标志物。它们的定量对于理解PG、iso-PG或氧化应激所涉及的许多生物过程是重要的。PG和iso-PG定量的常用方法之一是LC-MS/MS，该方法无需衍生化即可对前列腺素类化合物进行高选择性、灵敏的同时分析。然而，目前使用的LC-MS/MS方法需要多步提取和长时间(一小时内)的LC分离，才能实现对主要异构体PG的同时分离和分析。开发和验证的脑组织分析一步提取方案和UPLC-MS/MS方法显著提高了PG提取的回收率高达95%，并允许更快(在4分钟内)主要的异PGE2和-PGD2分离，与以前使用的方法相比，色谱峰窄了5倍。此外，由于在一次性离心管中执行一步提取方法，因此减少了分析时间和成本。总而言之，这大大提高了PG和iso-PG分析的灵敏度、时间和成本效率。
Prostaglandins (PG) and isoprostanes (iso-PG) may be derived through cyclooxygenase or free radical pathways and are important signaling molecules that are also robust biomarkers of oxidative stress. Their quantification is important for understanding many biological processes where PG, iso-PG, or oxidative stress are involved. One of the common methods for PG and iso-PG quantifications is LC-MS/MS that allows a highly selective, sensitive, simultaneous analysis for prostanoids without derivatization. However, the currently used LC-MS/MS methods require a multi-step extraction and a long (within an hour) LC separation to achieve simultaneous separation and analysis of the major iso-PG. The developed and validated for brain tissue analysis one-step extraction protocol and UPLC-MS/MS method significantly increases the recovery of the PG extraction up to 95%, and allows for a much faster (within 4 min) major iso-PGE2 and -PGD2 separation with 5 times narrower chromatographic peaks as compared to previously used methods. In addition, it decreases the time and cost of analysis due to one-step extraction approach performed in disposable centrifuge tubes. All together, this significantly increases the sensitivity, and the time and cost efficiency of the PG and iso-PG analysis.
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影响因子：
2
作者：
Masoodi, Mojgan;Nicolaou, Anna
通讯作者：
Nicolaou, Anna
影响因子：
2
作者：
Lee, SH;Williams, MV;Blair, IA
通讯作者：
Blair, IA
影响因子：
5.3
作者：
Metea, Monica R.;Kofuji, Paulo;Newman, Eric A.
通讯作者：
Newman, Eric A.
DOI：
10.1016/s1044-0305(02)00420-8
发表时间：
2002-07-01
影响因子：
3.2
作者：
Bateman, RH;Carruthers, R;Vissers, JPC
通讯作者：
Vissers, JPC
影响因子：
4.4
作者：
Austin, Susan A.;Rojanathammanee, Lalida;Combs, Colin K.
通讯作者：
Combs, Colin K.