Fluorescence intensity and intermittency as tools for following dopamine bioconjugate processing in living cells.
Fluorescence intensity and intermittency as tools for following dopamine bioconjugate processing in living cells.
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DOI:
10.1155/2007/70145
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发表时间:
2007
影响因子:
--
通讯作者:
Nadeau, Jay L.
中科院分区:
文献类型:
--
作者:
Khatchadourian, Rafael;Bachir, Alexia;Clarke, Samuel J.;Heyes, Colin D.;Wiseman, Paul W.;Nadeau, Jay L.
CdSe/ZnS quantum dots (QDs) conjugated to biomolecules that quench their fluorescence, particularly dopamine, have particular spectral properties that allow determination of the number of conjugates per particle, namely, photoenhancement and photobleaching. In this work, we quantify these properties on a single-particle and ensemble basis in order to evaluate their usefulness as a tool for indicating QD uptake, breakdown, and processing in living cells. This creates a general framework for the use of fluorescence quenching and intermittency to better understand nanoparticle-cell interactions.
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