Morphogenesis underlying the development of the everted teleost telencephalon.
Morphogenesis underlying the development of the everted teleost telencephalon.
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DOI：
10.1186/1749-8104-7-32
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发表时间：
2012-09-18
影响因子：
3.6
通讯作者：
Clarke JD
中科院分区：
文献类型：
--
作者：
Folgueira M;Bayley P;Navratilova P;Becker TS;Wilson SW;Clarke JD
尽管大脑模式和区域化的机制是非常保守的，但不同大脑区域的形态在脊椎动物的系统发育中是非常不同的。这一点在端脑上表现得尤为明显，在鳍鱼和其他脊椎动物之间的差异最为显著，鳍鱼的端脑是外翻的，而其他脊椎动物的端脑是外翻的。产生这些不同形态的机制尚不清楚。本研究通过分析斑马鱼受精后12小时（hpf）至受精后5天（dpf）前脑室的形成、基因和转基因表达的进化模式、神经元组织和命运定位，研究了斑马鱼端脑的形态发生。我们的研究结果突出了端脑形态发生的两个关键事件。首先，在端脑和间脑之间形成一个深脑室隐窝，即脑前内沟（AIS），有效地形成了一个通向端脑叶的后脑室壁。这一过程使端脑最后方的神经上皮区域向外侧移位。其次，随着端脑生长和神经发生在发育的第2天至第5天之间进行，端脑后脑室壁的苍白区凸起到AIS的背侧。这使得心室区（VZ）与AIS顶板接近，产生狭窄的心室空间和薄的脉管端（tc）。随着苍白区VZ的扩张，tc也在端脑的上表面扩张。在此期间，苍白部VZ的生长和延伸的主要轴是沿着前后轴。这第二步有效地以5 dpf的速度生成一个外翻的端脑。我们对端脑形态发生的描述挑战了传统的模型，即外翻仅仅是由于端脑神经上皮的侧向外折叠。这可能对理解成年鱼端脑的最终组织有重要意义。
Although the mechanisms underlying brain patterning and regionalization are very much conserved, the morphology of different brain regions is extraordinarily variable across vertebrate phylogeny. This is especially manifest in the telencephalon, where the most dramatic variation is seen between ray-finned fish, which have an everted telencephalon, and all other vertebrates, which have an evaginated telencephalon. The mechanisms that generate these distinct morphologies are not well understood. Here we study the morphogenesis of the zebrafish telencephalon from 12 hours post fertilization (hpf) to 5 days post fertilization (dpf) by analyzing forebrain ventricle formation, evolving patterns of gene and transgene expression, neuronal organization, and fate mapping. Our results highlight two key events in telencephalon morphogenesis. First, the formation of a deep ventricular recess between telencephalon and diencephalon, the anterior intraencephalic sulcus (AIS), effectively creates a posterior ventricular wall to the telencephalic lobes. This process displaces the most posterior neuroepithelial territory of the telencephalon laterally. Second, as telencephalic growth and neurogenesis proceed between days 2 and 5 of development, the pallial region of the posterior ventricular wall of the telencephalon bulges into the dorsal aspect of the AIS. This brings the ventricular zone (VZ) into close apposition with the roof of the AIS to generate a narrow ventricular space and the thin tela choroidea (tc). As the pallial VZ expands, the tc also expands over the upper surface of the telencephalon. During this period, the major axis of growth and extension of the pallial VZ is along the anteroposterior axis. This second step effectively generates an everted telencephalon by 5 dpf. Our description of telencephalic morphogenesis challenges the conventional model that eversion is simply due to a laterally directed outfolding of the telencephalic neuroepithelium. This may have significant bearing on understanding the eventual organization of the adult fish telencephalon.
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影响因子：
2.9
作者：
Mueller T;Dong Z;Berberoglu MA;Guo S
通讯作者：
Guo S
影响因子：
2.5
作者：
Butler, AB
通讯作者：
Butler, AB
影响因子：
2.5
作者：
Sheldon, RE
通讯作者：
Sheldon, RE
影响因子：
2.5
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
通讯作者：
SCHILLING, TF
影响因子：
2.5
作者：
Holmgren, N
通讯作者：
Holmgren, N