Use of the polymerase chain reaction to study arthritis due to Neisseria gonorrhoeae.
Use of the polymerase chain reaction to study arthritis due to Neisseria gonorrhoeae.
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使用聚合酶链反应研究淋病奈瑟菌引起的关节炎。
DOI：
10.1002/art.1780370515
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发表时间：
1994
影响因子：
--
通讯作者：
Steinman,CR
中科院分区：
文献类型：
--
作者：
Muralidhar,B;Rumore,PM;Steinman,CR
Objective.探讨聚合酶链反应（PCR）在淋病奈瑟菌性关节炎诊断、治疗和研究中的应用。应用PCR方法检测淋球菌感染模型系统和系统性淋球菌感染及关节炎患者滑液提取物中的DNA。在人类关节炎的SF中可检测到一种淋球菌或其等价物。8例系统性淋球菌感染和关节炎患者中有5例在至少1份淋球菌培养阳性的治疗前SF标本中PCR检测到淋球菌DNA。38份对照标本中有37份为阴性，例外情况可能是由于阳性标本的交叉污染。所有其他方法检测淋病奈瑟菌阳性的标本，PCR检测也为阳性。另2例PCR阳性，其他方法阴性。所有方法（包括PCR）均显示4份预处理标本为阴性。这表明，对于这些患者，阴性培养反映了真正的淋病奈瑟菌的缺乏，而不是存在不可培养的微生物。这组患者的病程也明显短于SF中发现淋病奈瑟菌DNA的患者。所有患者对抗生素治疗均迅速作出反应。3例标本阳性的患者中有2例治疗后SFN淋病DNA明显下降。使用这些或类似的寡核苷酸引物的PCR可以是一个有用的辅助诊断淋球菌性关节炎，并可以在评估其对治疗的反应的价值。在某些淋球菌相关性关节炎中，SF中似乎缺乏活的和非活的淋球菌。这些观察结果可能对这种形式的关节炎的发病机制有影响。
Objective. To explore the utility of the polymerase chain reaction (PCR) in the diagnosis, management, and investigation of arthritis due toNeisseria gonorrhoeae.Methods. The PCR was used to detect DNA fromN gonorrhoeaein model systems and in extracts of synovial fluid (SF) from patients with systemic gonococcal infections and objective evidence of arthritis.Results. OneN gonorrhoeaeorganism or its equivalent was detectable in human SF from inflamed joints. Five of 8 patients with systemicN gonorrhoeaeinfection and arthritis hadN gonorrhoeaeDNA demonstrated by PCR in at least 1 pretreatment SF specimen that wasN gonorrhoeaeculture positive. Thirty‐seven of 38 control specimens were negative, the exception probably being due to cross‐contamination from a positive specimen. All specimens that were positive forN gonorrhoeaeby other methods were also positive by PCR. Two others were positive by PCR but negative by other methods. Four pretreatment specimens were negative by all methods, including PCR. This suggests that, for these patients, negative cultures reflected true absence ofN gonorrhoeae, and not the presence of unculturable organisms. This group also had a significantly shorter duration of disease than did the patients withN gonorrhoeaeDNA found in their SF. All patients had a prompt response to antibiotic treatment. Two of 3 patients whose specimens were previously positive showed marked decreases in SFN gonorrhoeaeDNA after treatment.Conclusion. The PCR using these or similar oligonucleotide primers can be a useful adjunct in the diagnosis of gonococcal arthritis and can be of value in assessing its response to therapy. In someN gonorrhoeae–associated arthritides, there appears to be a lack of both viable and nonviableN gonorrhoeaeorganisms in the SF. These observations may have implications regarding the pathogenesis of this form of arthritis.
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源自核糖体 RNA 的特定淋病奈瑟氏菌 DNA 探针。
DOI：
--
发表时间：
1989
期刊：
Journal of General Microbiology
影响因子：
--
作者：
R. Rossau;E. Vanmechelen;J. D. Ley;H. V. Heuverswijn
通讯作者：
H. V. Heuverswijn
分子方法在传染病中的应用。
影响因子：
158.5
作者：
Lucy S. Tompkins
通讯作者：
Lucy S. Tompkins
影响因子：
158.5
作者：
RELMAN, DA;SCHMIDT, TM;FALKOW, S
通讯作者：
FALKOW, S
几何差异允许 PCR 产物和基因组靶标的不同酶失活。
影响因子：
3.5
作者：
Muralidhar,B;Steinman,CR
通讯作者：
Steinman,CR
影响因子：
158.5
作者：
RELMAN, DA;LOUTIT, JS;TOMPKINS, LS
通讯作者：
TOMPKINS, LS