Effect of spatial origin and hydrocarbon composition on bacterial consortia community structure and hydrocarbon biodegradation rates.
Effect of spatial origin and hydrocarbon composition on bacterial consortia community structure and hydrocarbon biodegradation rates.
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DOI：
10.1093/femsec/fiy127
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发表时间：
2018-09-01
影响因子：
4.2
通讯作者：
Witte U
中科院分区：
文献类型：
--
作者：
Potts LD;Perez Calderon LJ;Gontikaki E;Keith L;Gubry-Rangin C;Anderson JA;Witte U
目前正在加紧勘探深海沉积物中的石油储量，并伴随着石油溢漏的风险。研究表明，深海沉积物（> 1000 m）中的微生物群落具有降解碳氢化合物的能力，但其降解能力低于浅海沉积物（<200 m）中的微生物群落，这可能与温度有关。本研究旨在评估海洋来源对微生物HC降解潜力的影响，同时分离温度的影响，并研究与HC降解相关的细菌群落。来自135 m和1000 m深沉积物的微生物群落在原位温度下选择性地富集在原油上，随后将两种聚生体与两种HC混合物（柴油或模型油）在20°C下孵育42天。在这两个财团的存在下，HC的生物降解迅速发生，特别是低分子量的HC，并伴随着微生物群落的变化。此外，石油降解与浅财团比与深。优势HC降解菌不同的财团和补充HC类型的空间来源。本研究为沉积物空间来源和HC组成对海洋HC降解菌群的选择和活性的影响提供了证据，并为未来的生物修复发展提供了依据。本研究描述了海底沉积物来源对微生物碳氢化合物降解速率的影响以及不同碳氢化合物混合物对细菌聚生体组成的影响。
Oil reserves in deep-sea sediments are currently subject to intense exploration, with associated risks of oil spills. Previous research suggests that microbial communities from deep-sea sediment (>1000m) can degrade hydrocarbons (HCs), but have a lower degradation ability than shallow (<200m) communities, probably due to in situ temperature. This study aimed to assess the effect of marine origin on microbial HC degradation potential while separating the influence of temperature, and to characterise associated HC-degrading bacterial communities. Microbial communities from 135 and 1000 m deep sediments were selectively enriched on crude oil at in situ temperatures and both consortia were subsequently incubated for 42 days at 20°C with two HC mixtures: diesel fuel or model oil. Significant HC biodegradation occurred rapidly in the presence of both consortia, especially of low molecular weight HCs and was concomitant with microbial community changes. Further, oil degradation was higher with the shallow consortium than with the deep one. Dominant HC-degrading bacteria differed based on both spatial origin of the consortia and supplemented HC types. This study provides evidence for influence of sediment spatial origin and HC composition on the selection and activity of marine HC-degrading bacterial communities and is relevant for future bioremediationdevelopments. This study describes the effect of seabed sediment origin on microbial hydrocarbon degradation rates and the influence of different HC mixtures on bacterial consortia composition.
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影响因子：
3.6
作者：
Bagi, Andrea;Pampanin, Daniela M.;Kommedal, Roald
通讯作者：
Kommedal, Roald
影响因子：
3.4
作者：
Fasca H;de Castilho LVA;de Castilho JFM;Pasqualino IP;Alvarez VM;de Azevedo Jurelevicius D;Seldin L
通讯作者：
Seldin L
影响因子：
3.7
作者：
Brooks GR;Larson RA;Schwing PT;Romero I;Moore C;Reichart GJ;Jilbert T;Chanton JP;Hastings DW;Overholt WA;Marks KP;Kostka JE;Holmes CW;Hollander D
通讯作者：
Hollander D
影响因子：
3.7
作者：
Gutierrez T;Berry D;Yang T;Mishamandani S;McKay L;Teske A;Aitken MD
通讯作者：
Aitken MD
影响因子：
3.6
作者：
Guibert, Lilian M.;Loviso, Claudia L.;Lozada, Mariana
通讯作者：
Lozada, Mariana