Response of marine bacteria to oil contamination and to high pressure and low temperature deep sea conditions.
Response of marine bacteria to oil contamination and to high pressure and low temperature deep sea conditions.
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DOI：
10.1002/mbo3.550
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发表时间：
2018-04
期刊：
影响因子：
3.4
通讯作者：
Seldin L
中科院分区：
文献类型：
--
作者：
Fasca H;de Castilho LVA;de Castilho JFM;Pasqualino IP;Alvarez VM;de Azevedo Jurelevicius D;Seldin L
研究了压力和温度对石油烃污染海洋环境微生物群落的影响。本研究旨在揭示海洋细菌群落对低温，高压和石油烃污染的反应，使用巴西近海平台附近收集的海水样品。将仅含有海水的微宇宙和含有被1%原油污染的海水的微宇宙进行如下三种不同的温度和压力处理：（1）22°C/0.1 MPa;（2）4°C/0.1 MPa;和（3）4°C/22 MPa。还评估了减压后再加压对细菌群落的影响（4°C/22 MPaD）。通过PCR-DGGE和DNA测序分别分析了不同微宇宙中细菌群落的结构和组成。石油污染影响了在4°C或22°C和低压下培养的微宇宙中细菌群落的结构。即使在没有油污染的情况下，低温高压培养也会极大地影响细菌群落的结构。4°C/22 MPa和4°C/22 MPaD处理导致类似的DGGE曲线。DNA测序（40天的孵育后）显示，细菌属的多样性和相对丰度有关的存在或不存在的石油污染的非加压治疗。相比之下，在4°C/22 MPa的微观世界中，无论是否受到原油污染，细菌属的相对丰度的变化都不太明显。在4°C/22 MPa处理中观察到拟杆菌门的最高相对丰度。
The effect of pressure and temperature on microbial communities of marine environments contaminated with petroleum hydrocarbons is understudied. This study aims to reveal the responses of marine bacterial communities to low temperature, high pressure, and contamination with petroleum hydrocarbons using seawater samples collected near an offshore Brazilian platform. Microcosms containing only seawater and those containing seawater contaminated with 1% crude oil were subjected to three different treatments of temperature and pressure as follows: (1) 22°C/0.1 MPa; (2) 4°C/0.1 MPa; and (3) 4°C/22 MPa. The effect of depressurization followed by repressurization on bacterial communities was also evaluated (4°C/22 MPaD). The structure and composition of the bacterial communities in the different microcosms were analyzed by PCR‐DGGE and DNA sequencing, respectively. Contamination with oil influenced the structure of the bacterial communities in microcosms incubated either at 4°C or 22°C and at low pressure. Incubation at low temperature and high pressure greatly influenced the structure of bacterial communities even in the absence of oil contamination. The 4°C/22 MPa and 4°C/22 MPaD treatments resulted in similar DGGE profiles. DNA sequencing (after 40 days of incubation) revealed that the diversity and relative abundance of bacterial genera were related to the presence or absence of oil contamination in the nonpressurized treatments. In contrast, the variation in the relative abundances of bacterial genera in the 4°C/22 MPa‐microcosms either contaminated or not with crude oil was less evident. The highest relative abundance of the phylum Bacteroidetes was observed in the 4°C/22 MPa treatment.
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影响因子：
5.1
作者：
Grossi, Vincent;Yakimov, Michail M.;Tamburini, Christian
通讯作者：
Tamburini, Christian
影响因子：
11.4
作者：
Dubinsky, Eric A.;Conrad, Mark E.;Andersen, Gary L.
通讯作者：
Andersen, Gary L.
影响因子：
10.1
作者：
Joye, Samantha B.;Teske, Andreas P.;Kostka, Joel E.
通讯作者：
Kostka, Joel E.
影响因子：
2.9
作者：
Koyama, S;Kobayashi, H;Aizawa, M
通讯作者：
Aizawa, M
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影响因子：
5.2
作者：
Liu J;Bacosa HP;Liu Z
通讯作者：
Liu Z