Triterpenoid Saponins Isolated from Platycodon grandiflorum Inhibit Hepatitis C Virus Replication.
Triterpenoid Saponins Isolated from Platycodon grandiflorum Inhibit Hepatitis C Virus Replication.
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DOI：
10.1155/2013/560417
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Hwang SB
中科院分区：
文献类型：
--
作者：
Kim JW;Park SJ;Lim JH;Yang JW;Shin JC;Lee SW;Suh JW;Hwang SB
丙型肝炎病毒（HCV）感染是肝脏疾病的主要原因，包括肝硬化和肝细胞癌。由于目前开发的抗HCV药物具有明显的不良反应和耐药菌株的出现，植物提取物被认为是抗HCV新生物活性化合物的潜在来源。本研究旨在研究桔梗根提取物中三萜皂苷对HCV复制子细胞和HCV- (HCVcc-)感染细胞的病毒酶活性和复制的影响。通过NS5B RNA依赖性RNA聚合酶实验验证了PG三萜皂苷的抑制作用，并在HCV复制的背景下进一步证实了其抑制作用。6种三萜皂苷（桔梗素D、桔梗素D2、桔梗素D3、去桔梗素D、去桔梗素D2和桔梗酸A）和PG皂苷混合物（PGSM）均为抗hcv活性成分。重要的是，PGSM与干扰素-α或NS5A抑制剂联合发挥协同抗hcv活性。我们证明PGSM和IFN-α联合治疗有效地抑制了HCV耐药变体的集落形成。这些数据表明，三萜皂苷可能是一种新的抗hcv治疗剂。
Hepatitis C virus (HCV) infection is a major cause of liver disease, including cirrhosis and hepatocellular carcinoma. Due to significant adverse effects and emergence of resistant strains of currently developed anti-HCV agents, plant extracts have been considered to be potential sources of new bioactive compounds against HCV. The aim of this study was to evaluate the functional effects of triterpenoid saponins contained in the root extract of Platycodon grandiflorum (PG) on viral enzyme activities and replication in both HCV replicon cells and cell culture grown HCV- (HCVcc-) infected cells. Inhibitory activities of triterpenoid saponins from PG were verified by NS5B RNA-dependent RNA polymerase assay and were further confirmed in the context of HCV replication. Six triterpenoid saponins (platycodin D, platycodin D2, platycodin D3, deapioplatycodin D, deapioplatycodin D2, and platyconic acid A), PG saponin mixture (PGSM), were identified as active components exerting anti-HCV activity. Importantly, PGSM exerted synergistic anti-HCV activity in combination with either interferon-α or NS5A inhibitors. We demonstrated that combinatorial treatment of PGSM and IFN-α efficiently suppressed colony formation with significant reduction in drug resistant variant of HCV. These data suggest that triterpenoid saponin may represent a novel anti-HCV therapeutic agent.
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影响因子：
2.9
作者：
Choi, Jung Hoon;Yoo, Ki-Yeon;Kang, Il-Jun
通讯作者：
Kang, Il-Jun
影响因子：
4.9
作者：
Fridell, Robert A.;Qiu, Dike;Gao, Min
通讯作者：
Gao, Min
影响因子：
4.3
作者：
Lee, Kyung Jin;Choi, Jae Ho;Jeong, Hye Gwang
通讯作者：
Jeong, Hye Gwang
影响因子：
2.7
作者：
Lee, Hyun Yong;Yum, Ji Hyun;Hwang, Soon Bong
通讯作者：
Hwang, Soon Bong
DOI：
10.1248/yakushi1947.93.9_1188
发表时间：
1973-01-01
影响因子：
0.3
作者：
LEE, EB
通讯作者：
LEE, EB