Localization of photoperiod responsive circadian oscillators in the mouse suprachiasmatic nucleus.

Localization of photoperiod responsive circadian oscillators in the mouse suprachiasmatic nucleus.
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DOI:
10.1038/s41598-017-08186-5
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发表时间:
2017-08-15
期刊:
影响因子:
4.6
通讯作者:
Honma KI
Honma KI
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Yoshikawa T;Inagaki NF;Takagi S;Kuroda S;Yamasaki M;Watanabe M;Honma S;Honma KI

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视交叉上核(SCN)的昼夜节律起搏器产生光周期反应,将季节信息传递给生理和行为。为了确定参与SCN光周期反应的精确位置,我们分析了暴露于长或短白天的小鼠SCN水平切片中的昼夜节律Period 1和PERIOD 2。在像素水平上用各自的荧光素酶报告者对生物发光图像进行统计分析,使我们能够识别三个振荡区域的不同定位;一个大的开放环形区域,后端的区域和SCN中心的明显分界的椭圆形区域。前两个区域分别是所谓的夜间和早晨振荡器的位置,第三个区域可能是将光信号传递给前一个振荡器的位置。在这些区域中,有两类振荡细胞,其中Per 1和Per 2可以发挥不同的作用,在光周期反应。
The circadian pacemaker in the suprachiasmatic nucleus (SCN) yields photoperiodic response to transfer seasonal information to physiology and behavior. To identify the precise location involved in photoperiodic response in the SCN, we analyzed circadian Period1 and PERIOD2 rhythms in horizontally sectioned SCN of mice exposed to a long or short day. Statistical analyses of bioluminescence images with respective luciferase reporters on pixel level enabled us to identify the distinct localization of three oscillating regions; a large open-ring-shape region, the region at the posterior end and a sharply demarcated oval region at the center of the SCN. The first two regions are the respective sites for the so-called evening and morning oscillators, and the third region is possibly a site for mediating photic signals to the former oscillators. In these regions, there are two classes of oscillating cells in which Per1 and Per2 could play differential roles in photoperiodic responses.
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