NYVAC-Pf7: a poxvirus-vectored, multiantigen, multistage vaccine candidate for Plasmodium falciparum malaria
NYVAC-Pf7: a poxvirus-vectored, multiantigen, multistage vaccine candidate for Plasmodium falciparum malaria
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NYVAC-Pf7：一种针对恶性疟原虫疟疾的痘病毒载体、多抗原、多阶段候选疫苗
作者：
John A. Tine;D. Lanar;Darlene M. Smith;B. Wellde;Peter Schultheiss;L. Ware;Elizabeth B. Kauffman;R. Wirtz;C. Taisne;George Hui;Sandra P. Chang;Preston Church;MichaelR. Hollingdale;D. Kaslow;Stephen L. Hoffman;K. Guito;W. Ballou;Jerald Sadoff;Enzo Paoletti
高度减毒的NYVAC牛痘病毒株已被用于开发针对疟疾的多抗原、多阶段候选疫苗，疟疾是一种仍然是严重的全球健康问题并且不存在高效疫苗的疾病。将编码来自寄生虫生命周期的子孢子（环子孢子蛋白和子孢子表面蛋白2）、肝脏（肝脏阶段抗原1）、血液（裂殖子表面蛋白1、丝氨酸重复抗原和顶端膜抗原1）和性阶段（25-kDa性阶段抗原）的七种恶性疟原虫抗原的基因插入到单个NYVAC基因组中以产生NYVAC-Pf 7。在NYVAC-Pf 7感染的培养细胞中表达7种抗原中的每一种，并证明重组病毒的基因型和表型稳定性。当接种到恒河猴中时，NYVAC-Pf 7是安全的并且耐受性良好。抗体识别子孢子，肝脏，血液和性阶段的恶性疟原虫引起。针对恶性疟原虫抗原（环子孢子蛋白、子孢子表面蛋白2、裂殖子表面蛋白1和25-kDa性阶段抗原）的特异性抗体应答被表征。结果表明，NYVAC-Pf 7是用于在人类临床试验中进一步评价的合适的候选疫苗。
The highly attenuated NYVAC vaccinia virus strain has been utilized to develop a multiantigen, multistage vaccine candidate for malaria, a disease that remains a serious global health problem and for which no highly effective vaccine exists. Genes encoding seven Plasmodium falciparum antigens derived from the sporozoite (circumsporozoite protein and sporozoite surface protein 2), liver (liver stage antigen 1), blood (merozoite surface protein 1, serine repeat antigen, and apical membrane antigen 1), and sexual (25-kDa sexual-stage antigen) stages of the parasite life cycle were inserted into a single NYVAC genome to generate NYVAC-Pf7. Each of the seven antigens was expressed in NYVAC-Pf7-infected culture cells, and the genotypic and phenotypic stability of the recombinant virus was demonstrated. When inoculated into rhesus monkeys, NYVAC-Pf7 was safe and well tolerated. Antibodies that recognize sporozoites, liver, blood, and sexual stages of P. falciparum were elicited. Specific antibody responses against four of the P.falciparum antigens (circumsporozoite protein, sporozoite surface protein 2, merozoite surface protein 1, and 25-kDa sexual-stage antigen) were characterized. The results demonstrate that NYVAC-Pf7 is an appropriate candidate vaccine for further evaluation in human clinical trials.
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DOI：
10.1073/pnas.82.6.1785
发表时间：
1985-01-01
影响因子：
11.1
作者：
YEWDELL, JW;BENNINK, JR;MOSS, B
通讯作者：
MOSS, B
影响因子：
1.5
作者：
KLOTZ, FW;HADLEY, TJ;MILLER, LH
通讯作者：
MILLER, LH
使用表达 HSV 糖蛋白 D 的痘苗病毒重组体保护豚鼠免受原发性和复发性单纯疱疹病毒 (HSV) 2 型皮肤病的影响。
DOI：
10.1093/infdis/155.6.1188
发表时间：
1987
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Wachsman,M;Aurelian,L;Smith,CC;Lipinskas,BR;Perkus,ME;Paoletti,E
通讯作者：
Paoletti,E
恶性疟原虫：乌干达-帕洛阿尔托分离株主要裂殖子表面抗原 (gp195) 的基因结构和水病谱。
影响因子：
2.1
作者：
Chang,SP;Kramer,KJ;Yamaga,KM;Kato,A;Case,SE;Siddiqui,WA
通讯作者：
Siddiqui,WA