Forage crops alter soil bacterial and fungal communities in an apple orchard
Forage crops alter soil bacterial and fungal communities in an apple orchard
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饲料作物改变苹果园的土壤细菌和真菌群落
DOI：
10.1080/09064710.2015.1088569
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发表时间：
2016-04
期刊：
影响因子：
--
通讯作者：
Deguo Lyu
中科院分区：
文献类型：
--
作者：
Sijun Qin;Kuibao Jiao;Jiali He;Deguo Lyu
摘要本文研究了苹果果园种植三种牧草对土壤微生物群落结构的影响。苹果果园与本地草（NG）、红三叶草（RC;红车轴草L.）黑麦草（RE;三叶草（Trifolium repens L.），无植被（CT对照;清洁耕作）。我们从果园不同处理地块的0-20、20-40和40-60 cm深度获得土壤样品，并使用高通量DNA测序技术对其进行分析。 三种饲料作物对土壤细菌和真菌群落结构均有影响。与CT对照相比，NG间作提高了酸性细菌的比例，降低了硝化螺旋菌和疣微菌的比例，而RC间作提高了硝化螺旋菌和疣微菌的比例，降低了浮游菌的比例。间作稀土提高了硝化螺旋藻和绿球藻的比例，降低了酸杆菌的比例。与其他处理不同，间作NG增加了0-20 cm土层接合菌的比例。结果表明，间作RC增加了壶菌在3个土层中的比例，而间作RE增加了20-40 cm土层中担子菌的比例。总之，这些研究结果表明，间作与饲料作物，特别是RC，在苹果果园，可以改变土壤微生物群落结构。在前期的研究中，我们发现间作牧草改变了土壤微生物对单一碳源的利用，因此，通过改变微生物群落结构来提高苹果果园土壤碳循环效率是一种有效的途径。
ABSTRACT In this study, we investigated the effects of planting three types of forage crops in an apple orchard on the soil microbial community structure. The apple orchard was intercropped with native grasses (NG), red clover (RC; Trifolium pratense L.), ryegrass (RE; Trifolium repens L.), and no vegetation (CT control; clean tillage). We obtained soil samples from depths of 0–20, 20–40, and 40–60 cm from the different treatment plots in the orchard and analysed them using a high-throughput DNA sequencing technique. The three forage crops had affected the soil bacterial and fungal community structures. Compared with CT control, intercropping with NG increased the proportion of Acidobacteria and reduced those of Nitrospirae and Verrucomicrobia, whereas intercropping with RC increased the proportions of Nitrospirae and Verrucomicrobia and reduced that of Planctomycetes. Intercropping with RE increased the proportions of Nitrospirae and Chloroflexi, whereas reduced that of Acidobacteria. Furthermore, unlike in the other treatments, intercropping with NG increased the proportion of Zygomycota in the 0–20-cm soil layer. Intercropping with RC increased the proportion of Chytridiomycota in all the three soil layers, whereas intercropping with RE increased the proportion of Basidiomycota in the 20–40-cm soil layer. Collectively, these findings suggest that intercropping with forage crops, especially RC, in an apple orchard, could alter soil microbial community structure. In our previous study, we showed that microbial sole-carbon-source utilisation is changed by intercropping with forage crops; thus, it can be considered as an effective approach to improve the efficiency of soil C cycling in the apple orchard by altering the microbial community structure.
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影响因子：
9.7
作者：
S. Purin;O. K. Filho;S. L. Stürmer
通讯作者：
S. Purin;O. K. Filho;S. L. Stürmer
影响因子：
4.8
作者：
Strickland, Michael S.;Lauber, Christian;Bradford, Mark A.
通讯作者：
Bradford, Mark A.
影响因子：
3.6
作者：
Miethling, R;Wieland, G;Tebbe, CC
通讯作者：
Tebbe, CC
影响因子：
9.7
作者：
H. Høgh-Jensen;J. Schjoerring
通讯作者：
H. Høgh-Jensen;J. Schjoerring
影响因子：
9.7
作者：
Degens, BP;Harris, JA
通讯作者：
Harris, JA