Assessment of the immune responses induced in cattle after inoculation of a Mycobacterium bovis strain deleted in two mce2 genes.
Assessment of the immune responses induced in cattle after inoculation of a Mycobacterium bovis strain deleted in two mce2 genes.
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DOI：
10.1155/2012/258353
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发表时间：
2012
影响因子：
--
通讯作者：
Bigi F
中科院分区：
文献类型：
--
作者：
Blanco FC;Soria M;Gravisaco MJ;Bianco MV;Meikle V;Garbaccio S;Vagnoni L;Cataldi AA;Bigi F
针对牛结核病的有效候选疫苗的产生将有助于控制这种人畜共患疾病。通过敲除毒力基因产生的合理减毒牛分枝杆菌菌株是有希望的候选疫苗。然而，为了有效，这些候选疫苗应至少保持其毒力亲代牛支原体菌株的免疫学特性。因此，本研究的目的是获得 mce2 基因缺失的牛支原体菌株，并评估该突变对牛细菌引起的免疫学特征的影响。我们发现，接种突变株的牛体内 CD4+ T 细胞的激活与接种亲本株的动物的激活程度相当。此外，在体外刺激后，接种突变体的动物的外周血单核细胞产生比亲本菌株更高水平的 mRNA Th-1 细胞因子。因此，这些结果表明mce2突变体是一种有前途的牛结核病候选疫苗。
The generation of efficient candidate vaccines against bovine tuberculosis will contribute to the control of this zoonotic disease. Rationally attenuated Mycobacterium bovis strains generated by knockout of virulence genes are promising candidate vaccines. However, to be effective, these candidate vaccines should at least maintain the immunological properties of their virulent parental M. bovis strains. Therefore, the aim of this study was to obtain an M. bovis strain deleted in the mce2 genes and evaluate the effect of the mutation on the immunological profile elicited by the bacteria in cattle. We showed that the activation of CD4+ T cells in cattle inoculated with the mutant strain was equivalent to that in animals inoculated with the parental strain. Moreover, after in vitro stimulation, peripheral blood mononuclear cells from animals inoculated with the mutant produced higher levels of mRNA Th-1 cytokines than the parental strain. Therefore, these results indicate that the mce2 mutant is a promising candidate vaccine against bovine tuberculosis.
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影响因子：
4.4
作者：
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通讯作者：
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