Observing the invisible through imaging mass spectrometry, a window into the metabolic exchange patterns of microbes.
Observing the invisible through imaging mass spectrometry, a window into the metabolic exchange patterns of microbes.
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DOI：
10.1016/j.jprot.2012.05.036
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发表时间：
2012-08-30
影响因子：
3.3
通讯作者：
Dorrestein PC
中科院分区：
文献类型：
--
作者：
Gonzalez DJ;Xu Y;Yang YL;Esquenazi E;Liu WT;Edlund A;Duong T;Du L;Molnár I;Gerwick WH;Jensen PR;Fischbach M;Liaw CC;Straight P;Nizet V;Dorrestein PC
许多微生物可以作为单一物种群落培养。通常，这些菌落通过代谢物的分泌来控制和维持。这些代谢物是发现治疗药物（如青霉素、紫杉醇、雷帕霉素、埃博霉素）的宝贵资源。在这篇文章中，写了一个特殊的问题上成像质谱，我们表明，MALDI成像质谱可以适应观察，在空间上的方式，代谢交换模式的各种微生物，包括嗜热和嗜温真菌，蓝藻，海洋和陆地放线菌，和病原菌。取决于培养基条件，平均而言并基于手动分析，我们观察到与每个微生物IMS实验相关的11.3个分子，其在分泌分子和菌落相关分子之间的比例接近50：50。这些代谢交换因子的空间分布与生物体的生物学和生态学功能有关。这项工作确立了基于MALDI的IMS可以用作研究各种微生物的通用工具。此外，文章提出了IMS平台作为一个窗口的概念，发现以前未报道的分子，通过监测微生物在琼脂基质上生长时的代谢交换模式。
Many microbes can be cultured as single-species communities. Often, these colonies are controlled and maintained via the secretion of metabolites. Such metabolites have been an invaluable resource for the discovery of therapeutics (e.g. penicillin, taxol, rapamycin, epothilone). In this article, written for a special issue on imaging mass spectrometry, we show that MALDI-imaging mass spectrometry can be adapted to observe, in a spatial manner, the metabolic exchange patterns of a diverse array of microbes, including thermophilic and mesophilic fungi, cyanobacteria, marine and terrestrial actinobacteria, and pathogenic bacteria. Dependent on media conditions, on average and based on manual analysis, we observed 11.3 molecules associated with each microbial IMS experiment, which was split nearly 50:50 between secreted and colony-associated molecules. The spatial distributions of these metabolic exchange factors are related to the biological and ecological functions of the organisms. This work establishes that MALDI-based IMS can be used as a general tool to study a diverse array of microbes. Furthermore the article forwards the notion of the IMS platform as a window to discover previously unreported molecules by monitoring the metabolic exchange patterns of organisms when grown on agar substrates.
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影响因子：
15
作者：
Liu, Wei-Ting;Kersten, Roland D.;Yang, Yu-Liang;Moore, Bradley S.;Dorrestein, Pieter C.
通讯作者：
Dorrestein, Pieter C.
DOI：
10.1126/science.1176667
发表时间：
2009-08-28
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Fischbach MA;Walsh CT
通讯作者：
Walsh CT
影响因子：
1.8
作者：
Belofsky, GN;Jensen, PR;Fenical, W
通讯作者：
Fenical, W
DOI：
10.1038/ja.2010.22
发表时间：
2010-05
期刊：
The Journal of antibiotics
影响因子：
--
作者：
通讯作者：
--
影响因子：
48
作者：
Cornett, Dale S.;Reyzer, Michelle L.;Caprioli, Richard M.
通讯作者：
Caprioli, Richard M.