Isolation and characterization of new 24 microsatellite DNA markers for golden cuttlefish (Sepia esculenta).

Isolation and characterization of new 24 microsatellite DNA markers for golden cuttlefish (Sepia esculenta).
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金乌贼 (Sepia esculenta) 24 个新微卫星 DNA 标记的分离和表征

DOI:
10.3390/ijms13011154
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发表时间:
2012
影响因子:
5.6
通讯作者:
Zhuang Z
Zhuang Z
中科院分区:
生物学2区
文献类型:
--
作者:
Yuan Y;Liu S;Bai C;Liu H;Zhuang Z

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从富含(GT)13的基因组文库中分离并鉴定了24个金乌贼(Sepia esculenta)微卫星DNA标记。对胶州湾48个个体进行了基因座检测。每个位点的等位基因数为2 ~ 25个,平均10.3个。观察杂合度和期望杂合度的范围分别为0.063 ~ 0.896和0.137 ~ 0.953,平均值分别为0.519和0.633。经Bonferroni校正后,有6个位点显著偏离了Hardy-Weinberg平衡,位点间未发现明显的连锁不平衡。这些微卫星标记将有助于分析种群遗传结构,为保护和管理决策提供依据。食用植物
Twenty-four microsatellite DNA markers were isolated and characterized for golden cuttlefish (Sepia esculenta) from a (GT)13—enriched genomic library. Loci were tested in 48 individuals from Jiaozhou bay of China. The numbers of alleles per locus ranged from two to 25 with an average of 10.3. The observed and expected heterozygosities ranged from 0.063 to 0.896 and from 0.137 to 0.953, with averages of 0.519 and 0.633, respectively. Six loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni’s correction and no significant linkage disequilibrium between loci pairs was detected. These microsatellite markers would be useful for analyzing the population genetic structure to make conservation and management decisions for S. esculenta.
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