Development of new microsatellite DNA markers from Apostichopus japonicus and their cross-species application in Parastichopus parvimensis and Pathallus mollis.
Development of new microsatellite DNA markers from Apostichopus japonicus and their cross-species application in Parastichopus parvimensis and Pathallus mollis.
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仿刺参微卫星DNA新标记的开发及其在仿刺参和马齿苋中的跨物种应用
DOI：
10.3390/ijms12095862
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发表时间：
2011
影响因子：
5.6
通讯作者：
Chen G
中科院分区：
文献类型：
--
作者：
Liao M;Wang Y;Rong X;Zhang Z;Li B;Wang L;Chen G
利用20个微卫星DNA标记对60个中国野生仿刺参个体进行了多态性分析。结果表明，所有标记均表现出多态性。在20个位点上共检测到164个等位基因。每个位点的等位基因数在3 ~ 17个之间，平均为8.2个;每个位点的期望杂合度在0.03 ~ 0.89之间，平均为0.64。还在从美国收集的Parastichopus parvimensis和从秘鲁收集的Pathallus mollis中进行了跨种扩增。结果表明，有17个位点能扩增出Parastichopus parvimensis的DNA，只有4个位点能扩增出Pathallus mollis的DNA。这些多态性标记可用于海参的遗传育种和遗传变异的评估。
Twenty microsatellite DNA markers were developed for sea cucumber and used to investigate polymorphisms of 60 wild Apostichopus japonicus individuals collected from China. It revealed that all the markers were polymorphic. A total of 164 alleles were detected at 20 loci. The number of alleles per locus varied from 3 to 17 with an average of 8.2, and the expected heterozygosities of each locus ranged from 0.03 to 0.89 with an average of 0.64. Cross-species amplification was also conducted in Parastichopus parvimensis collected from the United States and Pathallus mollis collected from Peru. The result showed that 17 loci amplified Parastichopus parvimensis DNAs while only 4 loci could amplify Pathallus mollis DNAs. All of the polymorphic markers would be useful for future genetic breeding and the assessment of genetic variation within sea cucumbers.
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DOI：
10.3724/sp.j.1035.2008.00019
发表时间：
2008-01-01
期刊：
Acta Hydrobiologica Sinica
影响因子：
--
作者：
Zheng Jin-Song;Liao Xiao-Lin;Wang Ding
通讯作者：
Wang Ding
影响因子：
1.1
作者：
Sun, Wen-Jing;Li, Qi;Kong, Ling-Feng
通讯作者：
Kong, Ling-Feng
DOI：
10.1111/j.1471-8286.2007.01876.x
发表时间：
2007-11-01
期刊：
MOLECULAR ECOLOGY NOTES
影响因子：
--
作者：
Zhan, Aibin;Bao, Zhenmin;Hu, Jingjie
通讯作者：
Hu, Jingjie
影响因子：
4.4
作者：
WEBER, JL
通讯作者：
WEBER, JL
影响因子：
7.7
作者：
Rousset, Francois
通讯作者：
Rousset, Francois