Determinants of nucleosome organization in primary human cells.
Determinants of nucleosome organization in primary human cells.
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DOI：
10.1038/nature10002
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发表时间：
2011-05-22
期刊：
影响因子：
64.8
通讯作者：
Sidow, Arend
中科院分区：
文献类型：
--
作者：
Valouev, Anton;Johnson, Steven M.;Boyd, Scott D.;Smith, Cheryl L.;Fire, Andrew Z.;Sidow, Arend
Nucleosomes are the basic packaging units of chromatin, modulating accessibility of regulatory proteins to DNA and thus influencing eukaryotic gene regulation. Elaborate chromatin remodeling mechanisms have evolved that govern nucleosome organization at promoters, regulatory elements, and other functional regions in the genome. Analyses of chromatin landscape have uncovered a variety of mechanisms, including DNA sequence preferences, that can influence nucleosome positions. To identify major determinants of nucleosome organization in the human genome, we utilized deep sequencing to map nucleosome positions in three primary human cell types and in vitro. A majority of the genome exhibited substantial flexibility of nucleosome positions while a small fraction showed reproducibly positioned nucleosomes. Certain sites that position in vitro can anchor the formation of nucleosomal arrays that have cell type-specific spacing in vivo. Our results unveil an interplay of sequence-based nucleosome preferences and non-nucleosomal factors in determining nucleosome organization within mammalian cells.
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DOI：
10.1073/pnas.77.7.3816
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
TRIFONOV, EN;SUSSMAN, JL
通讯作者：
SUSSMAN, JL
影响因子：
7
作者：
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通讯作者：
Zhao, Keji
影响因子：
2.7
作者：
Radman-Livaja, Marta;Rando, Oliver J.
通讯作者：
Rando, Oliver J.
影响因子：
3.7
作者：
Tillo D;Kaplan N;Moore IK;Fondufe-Mittendorf Y;Gossett AJ;Field Y;Lieb JD;Widom J;Segal E;Hughes TR
通讯作者：
Hughes TR
影响因子：
4.4
作者：
Sha K;Gu SG;Pantalena-Filho LC;Goh A;Fleenor J;Blanchard D;Krishna C;Fire A
通讯作者：
Fire A