A decreased frequency of regulatory T cells in patients with common variable immunodeficiency.
A decreased frequency of regulatory T cells in patients with common variable immunodeficiency.
复制标题
常见可变免疫缺陷患者的调节性T细胞频率降低。
DOI：
10.1371/journal.pone.0006269
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发表时间：
2009-07-29
期刊：
影响因子：
3.7
通讯作者：
Costa-Carvalho BT
中科院分区：
文献类型：
--
作者：
Melo KM;Carvalho KI;Bruno FR;Ndhlovu LC;Ballan WM;Nixon DF;Kallas EG;Costa-Carvalho BT
常见变异性免疫缺陷障碍（CVID）是一种异质性综合征，其特征是抗体产生不足和复发性细菌感染以及T细胞异常。CD4+ cd25高调节性T细胞（Treg）是免疫应答的重要调节因子，包括下调对病原体、过敏原、癌细胞和自身抗原的免疫应答。在这项研究中，我们着手调查CVID患者中Treg细胞的频率及其与免疫激活状态的关系。16例常规静脉注射免疫球蛋白治疗的CVID患者（6男10女）和14例对照。采用多参数流式细胞术对外周血单个核细胞（PBMC）进行定量分析，使用以下细胞标志物：CD38、HLA-DR、CCR5（免疫活化）；CD4、CD25、FOXP3、CD127、OX40 （Treg细胞）；Ki-67和IFN-γ（细胞内细胞因子）。CVID患者CD4+CD25highFOXP3 T细胞比例明显低于健康对照组（P<0.05）。除了CVID患者的CD8+ T细胞中表达活化标志物CD38+和HLA-DR+的比例更高（P<0.05）外，我们发现Tregs与免疫活化之间没有显著相关性。我们的研究结果表明，Treg细胞的减少可能会损害CVID患者的免疫功能。
Common variable immunodeficiency disorder (CVID) is a heterogeneous syndrome, characterized by deficient antibody production and recurrent bacterial infections in addition abnormalities in T cells. CD4+CD25high regulatory T cells (Treg) are essential modulators of immune responses, including down-modulation of immune response to pathogens, allergens, cancer cells and self-antigens. In this study we set out to investigate the frequency of Treg cells in CVID patients and correlate with their immune activation status. Sixteen patients (6 males and 10 females) with CVID who had been treated with regular intravenous immunoglobulin and 14 controls were enrolled. Quantitative analyses of peripheral blood mononuclear cells (PBMC) were performed by multiparametric flow cytometry using the following cell markers: CD38, HLA-DR, CCR5 (immune activation); CD4, CD25, FOXP3, CD127, and OX40 (Treg cells); Ki-67 and IFN-γ (intracellular cytokine). A significantly lower proportion of CD4+CD25highFOXP3 T cells was observed in CVID patients compared with healthy controls (P<0.05). In addition to a higher proportion of CD8+ T cells from CVID patients expressing the activation markers, CD38+ and HLA-DR+ (P<0.05), we observed no significant correlation between Tregs and immune activation. Our results demonstrate that a reduction in Treg cells could have impaired immune function in CVID patients.
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表达CD4+CD25高T调节细胞的CD127LOW与HTLV-1感染中的人类T淋巴营养性病毒类型1（HTLV-1）型病毒型和HTLV-1相关的骨髓性骨髓性痉挛/热带痉挛性帕帕拉斯（HTLV-1）的频率成反比。
影响因子：
3
作者：
Michaelsson, Jakob;Barbosa, Hugo Marcelo R.;Jordan, Kimberley A.;Chapman, Joan M.;Brunialti, Milena K. C.;Neto, Walter Kleine;Nukui, Youko;Sabino, Ester C.;Chieia, Marco Antonio;Bulle Oiliveira, Acary Souza;Nixon, Douglas F.;Kallas, Esper G.
通讯作者：
Kallas, Esper G.
CD127表达与FOXP3和人类CD4+ T Reg细胞的抑制功能成反比。
DOI：
10.1084/jem.20060772
发表时间：
2006-07-10
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
通讯作者：
--
影响因子：
9.1
作者：
Lopes-da-Silva, Susana;Rizzo, Luiz Vicente
通讯作者：
Rizzo, Luiz Vicente
影响因子：
8.6
作者：
Cunningham-Rundles, C;Bodian, C
通讯作者：
Bodian, C
影响因子：
4.6
作者：
Kondratenko, I;Amlot, PL;Farrant, J
通讯作者：
Farrant, J