Staphylococcus aureus CidC Is a Pyruvate:Menaquinone Oxidoreductase.
Staphylococcus aureus CidC Is a Pyruvate:Menaquinone Oxidoreductase.
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DOI：
10.1021/acs.biochem.7b00570
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发表时间：
2017-09-12
期刊：
影响因子：
2.9
通讯作者：
Luca S
中科院分区：
文献类型：
--
作者：
Zhang X;Bayles KW;Luca S
Recent studies have revealed an important role for the Staphylococcus aureus CidC enzyme in cell death during the stationary phase and in biofilm development and have contributed to our understanding of the metabolic processes that are important in the induction of bacterial programmed cell death (PCD). To gain more insight into the characteristics of this enzyme, we performed an in-depth biochemical and biophysical analysis of its catalytic properties. In vitro experiments show that this flavoprotein catalyzes the oxidative decarboxylation of pyruvate to acetate and carbon dioxide. CidC efficiently reduces menadione, but not CoenzymeQ0, suggesting a specific role in the S. aureus respiratory chain. CidC exists as a monomer under neutral-pH conditions but tends to aggregate and bind to artificial lipid membranes at acidic pH, resulting in enhanced enzymatic activity. Unlike its Escherichia coli counterpart, PoxB, CidC does not appear to be activated by other amphiphiles like Triton X-100 or octyl β-d-glucopyranoside. In addition, only reduced CidC is protected from proteolytic cleavage by chymotrypsin, and unlike its homologues in other bacteria, protease treatment does not increase CidC enzymatic activity. Finally, CidC exhibits maximal activity at pH 5.5–5.8 and negligible activity at pH 7–8. The results of this study are consistent with a model in which CidC functions as a pyruvate:menaquinone oxidoreductase whose activity is induced at the cellular membrane during cytoplasmic acidification, a process previously shown to be important for the induction of bacterial PCD.
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影响因子：
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作者：
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