Whole-Mount Adult Ear Skin Imaging Reveals Defective Neuro-Vascular Branching Morphogenesis in Obese and Type 2 Diabetic Mouse Models.
Whole-Mount Adult Ear Skin Imaging Reveals Defective Neuro-Vascular Branching Morphogenesis in Obese and Type 2 Diabetic Mouse Models.
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DOI：
10.1038/s41598-017-18581-7
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发表时间：
2018-01-11
影响因子：
4.6
通讯作者：
Mukouyama YS
中科院分区：
文献类型：
--
作者：
Yamazaki T;Li W;Yang L;Li P;Cao H;Motegi SI;Udey MC;Bernhard E;Nakamura T;Mukouyama YS
Obesity and type 2 diabetes are frequently associated with peripheral neuropathy. Though there are multiple methods for diagnosis and analysis of morphological changes of peripheral nerves and blood vessels, three-dimensional high-resolution imaging is necessary to appreciate the pathogenesis with an anatomically recognizable branching morphogenesis and patterning. Here we established a novel technique for whole-mount imaging of adult mouse ear skin to visualize branching morphogenesis and patterning of peripheral nerves and blood vessels. Whole-mount immunostaining of adult mouse ear skin showed that peripheral sensory and sympathetic nerves align with large-diameter blood vessels. Diet-induced obesity (DIO) mice exhibit defective vascular smooth muscle cells (VSMCs) coverage, while there is no significant change in the amount of peripheral nerves. The leptin receptor-deficient db/db mice, a severe obese and type 2 diabetic mouse model, exhibit defective VSMC coverage and a large increase in the amount of smaller-diameter nerve bundles with myelin sheath and unmyelinated nerve fibers. Interestingly, an increase in the amount of myeloid immune cells was observed in the DIO but not db/db mouse skin. These data suggest that our whole-mount imaging method enables us to investigate the neuro-vascular and neuro-immune phenotypes in the animal models of obesity and diabetes.
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DOI：
10.1038/oby.2010.1
发表时间：
2010-10
期刊：
Obesity (Silver Spring, Md.)
影响因子：
--
作者：
Strissel KJ;DeFuria J;Shaul ME;Bennett G;Greenberg AS;Obin MS
通讯作者：
Obin MS
影响因子：
2.5
作者：
Li, Wenling;Mukouyama, Yoh-suke
通讯作者：
Mukouyama, Yoh-suke
影响因子：
64.5
作者：
Mukouyama, Y;Shin, D;Anderson, DJ
通讯作者：
Anderson, DJ
影响因子：
64.5
作者：
Chen, H;Charlat, O;Morgenstern, JP
通讯作者：
Morgenstern, JP
影响因子：
11.8
作者：
Li, Wenling;Kohara, Hiroshi;Uchida, Yutaka;James, Jennifer M.;Soneji, Kosha;Cronshaw, Darran G.;Zou, Yong-Rui;Nagasawa, Takashi;Mukouyama, Yoh-suke
通讯作者：
Mukouyama, Yoh-suke