Electrochemical quantification of serotonin in the live embryonic zebrafish intestine.
Electrochemical quantification of serotonin in the live embryonic zebrafish intestine.
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DOI：
10.1021/ac902465v
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发表时间：
2010-03-01
影响因子：
7.4
通讯作者：
Andreescu, Silvana
中科院分区：
文献类型：
--
作者：
Njagi, John;Ball, Michael;Best, Marc;Wallace, Kenneth N.;Andreescu, Silvana
我们使用差分脉冲伏安法，通过植入碳纤维微电极（碳纳米管分散在nafion中），实时监测发育早期（5 dpf）完整斑马鱼胚胎消化系统中5-羟色胺释放的体内水平。检测限为1 nM，线性范围为5 - 200 nM，灵敏度为83.65 nA·μM−1。将微电极植入斑马鱼胚胎肠道的不同位置。在正常生理条件下，体内测得血清素水平高达29.9（±1.13）nM。在完整的活胚胎中进行测量，除电极插入外没有额外的干扰。该传感器能够量化5-羟色胺释放的药理学改变，并以高空间分辨率提供这种神经递质沿着肠道的纵向分布。在存在氟伏沙明（一种选择性5-羟色胺再摄取抑制剂（SSRI））的情况下，记录的浓度为54.1（±1.05）nM，而在存在对氯苯丙氨酸（PCPA）（一种色氨酸羟化酶抑制剂）的情况下，5-羟色胺水平降至7.2（±0.45）nM。血清5-羟色胺水平的变化与免疫组化分析相关。我们已经证明了第一次使用的电化学微传感器在体内监测完整的斑马鱼胚胎的肠道血清素水平。
We monitored real-time in vivo levels of serotonin release in the digestive system of intact zebrafish embryos during early development (5 dpf) using differential pulse voltammetry with implanted carbon fiber microelectrodes modified with carbon nanotubes dispersed in nafion. A detection limit of 1 nM, a linear range between 5 to 200 nM and a sensitivity of 83.65 nA·μM−1 were recorded. The microelectrodes were implanted at various locations in the intestine of zebrafish embryos. Serotonin levels of up to 29.9(±1.13) nM were measured in vivo in normal physiological conditions. Measurements were performed in intact live embryos without additional perturbation beyond electrode insertion. The sensor was able to quantify pharmacological alterations in serotonin release and provide the longitudinal distribution of this neurotransmitter along the intestine with high spatial resolution. In the presence of fluvoxamine, a selective serotonin reuptake inhibitor (SSRI), concentrations of 54.1(±1.05) nM were recorded while in the presence of p-chloro-phenylalanine (PCPA), a tryptophan hydroxylase inhibitor, the serotonin levels decreased to 7.2(±0.45) nM. The variation of serotonin levels was correlated with immunohistochemical analysis. We have demonstrated the first use of electrochemical microsensors for in vivo monitoring of intestinal serotonin levels in intact zebrafish embryos.
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影响因子：
3.3
作者：
GERSHON, MD;TAMIR, H
通讯作者：
TAMIR, H
影响因子：
3.2
作者：
Miwa, J;Echizen, H;Umeda, N
通讯作者：
Umeda, N
影响因子：
15.9
作者：
Kim, M;Javed, NH;Cooke, HJ
通讯作者：
Cooke, HJ
DOI：
10.1016/0165-1838(93)90360-7
发表时间：
1993-10-01
期刊：
JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM
影响因子：
--
作者：
BLACKSHAW, LA;GRUNDY, D
通讯作者：
GRUNDY, D
DOI：
10.1152/ajpgi.1998.275.3.g433
发表时间：
1998-09-01
影响因子：
4.5
作者：
Chen, JX;Pan, H;Gershon, MD
通讯作者：
Gershon, MD