Effect of masticatory stimulation on the quantity and quality of saliva and the salivary metabolomic profile.
Effect of masticatory stimulation on the quantity and quality of saliva and the salivary metabolomic profile.
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DOI：
10.1371/journal.pone.0183109
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Kimoto K
中科院分区：
文献类型：
--
作者：
Okuma N;Saita M;Hoshi N;Soga T;Tomita M;Sugimoto M;Kimoto K
本研究旨在探讨咀嚼刺激对唾液质和量的影响，以及唾液代谢组学的变化。从55名受试者中收集刺激和未刺激的唾液样本，并使用毛细管电泳-飞行时间质谱法对唾液亲水性代谢物进行全面定量。总共鉴定和定量了137种代谢物。刺激唾液中44种代谢物的浓度显著高于非刺激唾液。途径分析确定了尿素循环的上调和甘氨酸，丝氨酸，半胱氨酸和苏氨酸在刺激唾液中的合成和降解途径。主成分分析显示，咀嚼刺激对唾液代谢谱的影响较少依赖于样本人群的性别，年龄和吸烟。非刺激唾液中仅1种代谢物的浓度和刺激唾液中的3种代谢物的浓度与唾液分泌量显著相关，表明唾液代谢组学特征和唾液分泌量是独立因素。咀嚼刺激不仅影响唾液分泌量，但代谢产物的浓度模式。分泌量和这些模式之间的低相关性支持的结论是，唾液代谢组学档案可能是一个新的指标来表征咀嚼刺激。
This study characterized the changes in quality and quantity of saliva, and changes in the salivary metabolomic profile, to understand the effects of masticatory stimulation. Stimulated and unstimulated saliva samples were collected from 55 subjects and salivary hydrophilic metabolites were comprehensively quantified using capillary electrophoresis-time-of-flight mass spectrometry. In total, 137 metabolites were identified and quantified. The concentrations of 44 metabolites in stimulated saliva were significantly higher than those in unstimulated saliva. Pathway analysis identified the upregulation of the urea cycle and synthesis and degradation pathways of glycine, serine, cysteine and threonine in stimulated saliva. A principal component analysis revealed that the effect of masticatory stimulation on salivary metabolomic profiles was less dependent on sample population sex, age, and smoking. The concentrations of only 1 metabolite in unstimulated saliva, and of 3 metabolites stimulated saliva, showed significant correlation with salivary secretion volume, indicating that the salivary metabolomic profile and salivary secretion volume were independent factors. Masticatory stimulation affected not only salivary secretion volume, but also metabolite concentration patterns. A low correlation between the secretion volume and these patterns supports the conclusion that the salivary metabolomic profile may be a new indicator to characterize masticatory stimulation.
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