The lipid composition of isolated cytoplasmic lipid droplets from a human cancer cell line, BE(2)M17.
The lipid composition of isolated cytoplasmic lipid droplets from a human cancer cell line, BE(2)M17.
复制标题
从人类癌细胞系 BE(2)M17 中分离出的细胞质脂滴的脂质成分。
DOI：
10.1039/c2mb05485j
复制
发表时间：
2012
影响因子：
--
通讯作者：
Pan X
中科院分区：
文献类型：
--
作者：
Pan X
肿瘤中脂质的~1H核磁共振波谱与肿瘤分级和治疗反应有关。这些核磁共振信号的来源主要被认为是细胞质脂滴(LDS)。目前已有分离低密度脂蛋白的技术，但对其组成及其与核磁共振信号的关系知之甚少。在这项工作中，密度梯度超速离心法在匀浆的人类癌细胞中分离LDS。对全细胞、分离的LDS及其提取物进行了核磁共振氢谱分析。采用异核单量子相干光谱(HSQC)和液-质联用(LC-MS)对丹参的脂类提取物进行了分析。用染色和显微镜观察分离LDS的形态。在化学位移和相对信号强度方面，细胞内的脂类共振和分离的LD光谱之间观察到了很好的一致性，支持核磁共振可见脂类主要来自LDS。分离的LDS显示高浓度的不饱和脂类，油酸与亚油酸的比率大于2，胆固醇酯(ChE)与胆固醇(CH)的比率接近1。在整个细胞中，这些比率比各自的比率大几倍，表明分离对于鉴定LD的组成是重要的。低密度脂蛋白含有一组特殊的脂类物种，可能对细胞的~1H核磁共振谱有贡献。
1H nuclear magnetic resonance spectroscopy (NMR) resonances from lipids in tumours are associated with tumour grade and treatment response. The origin of these NMR signals is mainly considered to be cytoplasmic lipid droplets (LDs). Techniques exist for isolating LDs but little is known about their composition and its relationship to NMR signals. In this work, density-gradient ultracentrifugation was performed on homogenised human cancer cells to isolate LDs. 1H NMR was performed on whole cells, isolated LDs and their extracts. Heteronuclear single quantum coherence spectroscopy (HSQC) and liquid chromatography mass spectroscopy (LC-MS) were performed on lipid extracts of LDs. Staining and microscopy were used to characterize isolated LDs. An excellent agreement in chemical shift and relative signal intensity was observed between lipid resonances in cells and isolated LD spectra supporting that NMR-visible lipids originate primarily from LDs. Isolated LDs showed high concentrations of unsaturated lipids, a oleic-to-linoleic acid ratio greater than two and a cholesteryl ester (ChE)-to-cholesterol (Ch) ratio close to unity. These ratios were several-fold greater than respective ratios in whole cells, demonstrating isolation is important to characterize LD composition. LDs contain a specific group of lipid species that are likely to contribute to the 1H NMR spectrum of cells.
登录
查看更多内容
DOI：
10.1007/s00249-002-0273-8
发表时间：
2003-05-01
影响因子：
2
作者：
Ferretti, A;Knijn, A;Sargiacomo, M
通讯作者：
Sargiacomo, M
通过多核磁共振波谱表征人脑组织的脂质。
影响因子：
2.9
作者：
V. Tugnoli;M. Tosi;A. Tinti;A. Trinchero;G. Bottura;G. Fini
通讯作者：
G. Fini
影响因子：
6.1
作者：
Qi Zhi-gang;Li Yu-xin;Chen Xin-rong
通讯作者：
Chen Xin-rong
影响因子：
2.9
作者：
KUESEL, AC;SUTHERLAND, GR;SMITH, ICP
通讯作者：
SMITH, ICP
影响因子：
8.8
作者：
通讯作者：
--