Pimonidazole binding in C6 rat brain glioma: relation with lipid droplet detection.
Pimonidazole binding in C6 rat brain glioma: relation with lipid droplet detection.
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DOI：
10.1038/sj.bjc.6600837
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发表时间：
2003-05-06
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
作者：
在C6大鼠脑胶质瘤中，我们研究了缺氧与坏死附近活细胞细胞质中脂滴存在的关系。为此，将大鼠立体定向植入C6细胞。实验是在肿瘤发展末期进行的。采用多荧光染色方案结合数字图像分析，定量研究了单冷冻切片中缺氧细胞（pimonidazole）、血液灌注（Hoechst 33342）、总血管床（collagen type IV）和脂滴（Red Oil）的空间分布。所有肿瘤（n=6）均表现为坏死、吡莫硝唑结合及脂滴。吡咪唑的结合发生在距离灌注血管平均114 μm处，主要发生在坏死周围。脂滴主要位于坏死组织中。在坏死周围的部分吡硝唑结合细胞中也观察到一些较小的液滴。因此，脂滴仅出现在坏死附近的缺氧细胞中，距离灌注血管约181 μm。总之，我们的结果表明，严重缺氧的细胞积累了小脂滴。然而，不存在100%的缺氧和脂滴共定位。因此，脂滴不能被认为是缺氧的替代标志，而是严重的坏死前缺氧的替代标志。
In C6 rat brain glioma, we have investigated the relation between hypoxia and the presence of lipid droplets in the cytoplasm of viable cells adjacent to necrosis. For this purpose, rats were stereotaxically implanted with C6 cells. Experiments were carried out by the end of the tumour development. A multifluorescence staining protocol combined with digital image analysis was used to quantitatively study the spatial distribution of hypoxic cells (pimonidazole), blood perfusion (Hoechst 33342), total vascular bed (collagen type IV) and lipid droplets (Red Oil) in single frozen sections. All tumours (n=6) showed necrosis, pimonidazole binding and lipid droplets. Pimonidazole binding occurred at a mean distance of 114 μm from perfused vessels mainly around necrosis. Lipid droplets were principally located in the necrotic tissue. Some smaller droplets were also observed in part of the pimonidazole-binding cells surrounding necrosis. Hence, lipid droplets appeared only in hypoxic cells adjacent to necrosis, at an approximate distance of 181 μm from perfused vessels. In conclusion, our results show that severe hypoxic cells accumulated small lipid droplets. However, a 100% colocalisation of hypoxia and lipid droplets does not exist. Thus, lipid droplets cannot be considered as a surrogate marker of hypoxia, but rather of severe, prenecrotic hypoxia.
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DOI：
10.1016/s0022-2828(85)80077-8
发表时间：
1985-01-01
影响因子：
5
作者：
JODALEN, H;STANGELAND, L;LEKVEN, J
通讯作者：
LEKVEN, J
DOI：
10.1016/s0360-3016(96)00539-1
发表时间：
1997-03-01
影响因子：
7
作者：
Kennedy, AS;Raleigh, JA;Varia, MA
通讯作者：
Varia, MA
影响因子：
12.7
作者：
MORIOKA, T;BABA, T;STREIT, WJ
通讯作者：
STREIT, WJ
影响因子：
4.1
作者：
Negendank, WG;Sauter, R;Zimmerman, RA
通讯作者：
Zimmerman, RA
影响因子：
3.1
作者：
RIJKEN, PFJW;BERNSEN, HJJA;VANDERKOGEL, AJ
通讯作者：
VANDERKOGEL, AJ