A photo-electrochemical aptasensor for the determination of severe acute respiratory syndrome coronavirus 2 receptor-binding domain by using graphitic carbon nitride-cadmium sulfide quantum dots nanocomposite.
A photo-electrochemical aptasensor for the determination of severe acute respiratory syndrome coronavirus 2 receptor-binding domain by using graphitic carbon nitride-cadmium sulfide quantum dots nanocomposite.
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DOI：
10.1016/j.snb.2021.130377
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发表时间：
2021-10-15
期刊：
影响因子：
--
通讯作者：
Acedo P
中科院分区：
文献类型：
--
作者：
Amouzadeh Tabrizi M;Nazari L;Acedo P
Aptamer/Chitosan/CdS QDs-gC 3 N4/ITO电极检测SARS冠状病毒2-RBD的响应机制示意图。本文首次报道了一种用于定量检测SARS冠状病毒2型受体结合域（Sars-Cov-2 RBD）的光电化学适体传感器。为此，首先，石墨氮化碳和（gC 3 N4）和硫化镉（CdS）量子点的制造和表征。然后，将gC 3 N4和CdS充分混合。所制造的纳米材料，其特征在于通过扫描透射电子显微镜。然后，将CdS QDs-gC 3 N4纳米复合材料添加到含有作为富含胺的聚合物的壳聚糖的溶液中以产生壳聚糖/CdS-gC 3 N4纳米复合材料。随后，用壳聚糖/CdS-gC 3 N4修饰ITO电极的表面。然后，以戊二醛为胺-胺交联剂，将氨基末端适配体探针固定在壳聚糖/CdS QDs-gC 3 N4/ITO电极表面。采用循环伏安法（CV）、电化学阻抗谱（EIS）和光电化学（PEC）研究了电极的电化学性能。发现固定的适体探针的表面覆盖度为26.2pmol.cm-2。所获得的结果表明，所提出的光电化学适体传感器可用于在0.5 - 32.0 nM范围内测量Sars-Cov-2 RBD。检测限（LOD）为0.12 nM（3σ/斜率）。通过使用Langmuir典型吸附系统，还发现适体/壳聚糖/CdS QDs-gC 3 N4/ITO的亲和力为3.4nM。建议的光电化学适体传感器被应用于测量在两个浓度的人唾液样品中的加标Sars-Cov-2 RBD。还研究了干扰生物材料如人免疫球蛋白G、人免疫球蛋白A、人免疫球蛋白M和人血清白蛋白的作用。
Schematic diagram of the response mechanism of PEC detection of Sars-Cov-2-RBD by using Aptamer/Chitosan/CdS QDs-gC3N4/ITO electrode. Herein, a photoelectrochemical aptasensor for the quantitive measurement of the severe acute respiratory syndrome coronavirus-2 receptor-binding domain (Sars-Cov-2 RBD) has been reported for the first time. For this purpose, first, graphitic carbon nitride and (gC3N4) and cadmium sulfide (CdS) quantum dots were fabricated and characterized. After that, gC3N4 and CdS were mixed well. The fabricated nanomaterials were characterized by scanning transmission electron microscopy. Then, the CdS QDs-gC3N4 nanocomposite was added to the solution containing chitosan as an amine-rich polymer to generate a Chitosan/CdS-gC3N4 nanocomposite. Subsequently, the surface of the ITO electrode was modified with Chitosan/CdS-gC3N4. After that, the amine-terminal aptamer probes were immobilized on the surface of the Chitosan/CdS QDs-gC3N4/ITO electrode by using glutaraldehyde as an amine-amine crosslinker. The electrochemical performances of the electrodes were studied using cyclic voltammetry (CV), electrochemical Impedance Spectroscopy (EIS), and photo-electrochemistry (PEC). The surface coverage of the immobilized aptamer probe was founded to be 26.2 pmol.cm−2. The obtained results demonstrated that the proposed photo-electrochemical aptasensor can be used for the measurement of Sars-Cov-2 RBD within 0.5−32.0 nM. The limit of detection (LOD) was obtained to be 0.12 nM (at 3σ/slope). The affinity of the Aptamer/Chitosan/CdS QDs-gC3N4/ITO was also founded to be 3.4 nM by using Langmuir-typical adsorption systems. The proposed photo-electrochemical aptasensor was applied for the measurement of the spiked Sars-Cov-2 RBD in human saliva samples at two concentrations. The effect of the interfering biomaterials such as human immunoglobulin G human immunoglobulin A, human immunoglobulin M, and human serum albumin was also studied.
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