Biomechanics of the chick embryonic heart outflow tract at HH18 using 4D optical coherence tomography imaging and computational modeling.
Biomechanics of the chick embryonic heart outflow tract at HH18 using 4D optical coherence tomography imaging and computational modeling.
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DOI：
10.1371/journal.pone.0040869
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Rugonyi S
中科院分区：
文献类型：
--
作者：
Liu A;Yin X;Shi L;Li P;Thornburg KL;Wang R;Rugonyi S
在发育阶段，对心脏细胞的生物力学刺激可调节遗传程序，偏离正常刺激可导致心脏缺陷。因此，在早期发育阶段表征正常的心脏生物力学刺激是很重要的。利用鸡胚胎心脏发育模型，我们重点研究了汉堡-汉密尔顿（HH） 18鸡心脏流出道（OFT）的生物力学刺激特征，这是人类观察到的大部分缺陷起源于心脏的远端部分。为了表征OFT中的生物力学刺激，我们结合了体内光学相干断层扫描（OCT）成像、生理测量和计算流体动力学（CFD）建模。我们发现，在HH18时，OFT壁近端比远端承受更大的周向应变，而OFT壁远端承受更大的壁应力。最大的壁剪切应力通常出现在心内膜垫的表面，心内膜垫是细胞外基质在OFT管腔上的突出物，在发育后期形成心间隔和瓣膜。OFT壁应力和应变的非均匀时空分布为心肌细胞提供了生物力学线索，这可能有助于在正常发育过程中观察到的广泛的差异生长和重塑模式。
During developmental stages, biomechanical stimuli on cardiac cells modulate genetic programs, and deviations from normal stimuli can lead to cardiac defects. Therefore, it is important to characterize normal cardiac biomechanical stimuli during early developmental stages. Using the chicken embryo model of cardiac development, we focused on characterizing biomechanical stimuli on the Hamburger–Hamilton (HH) 18 chick cardiac outflow tract (OFT), the distal portion of the heart from which a large portion of defects observed in humans originate. To characterize biomechanical stimuli in the OFT, we used a combination of in vivo optical coherence tomography (OCT) imaging, physiological measurements and computational fluid dynamics (CFD) modeling. We found that, at HH18, the proximal portion of the OFT wall undergoes larger circumferential strains than its distal portion, while the distal portion of the OFT wall undergoes larger wall stresses. Maximal wall shear stresses were generally found on the surface of endocardial cushions, which are protrusions of extracellular matrix onto the OFT lumen that later during development give rise to cardiac septa and valves. The non-uniform spatial and temporal distributions of stresses and strains in the OFT walls provide biomechanical cues to cardiac cells that likely aid in the extensive differential growth and remodeling patterns observed during normal development.
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影响因子：
3.5
作者：
Liu, Aiping;Wang, Ruikang;Rugonyi, Sandra
通讯作者：
Rugonyi, Sandra
影响因子：
--
作者：
MARKWALD, RR;FITZHARRIS, TP;MANASEK, FJ
通讯作者：
MANASEK, FJ
DOI：
10.1161/01.atv.0000143855.85343.0e
发表时间：
2004-11-01
影响因子：
8.7
作者：
Dancu, MB;Berardi, DE;Tarbell, JM
通讯作者：
Tarbell, JM
影响因子：
3.6
作者：
LEATHERBURY, L;CONNUCK, DM;KIRBY, ML
通讯作者：
KIRBY, ML
影响因子：
20.1
作者：
KELLER, BB;HU, N;CLARK, EB
通讯作者：
CLARK, EB