Enhanced synergistic effects from multiple iron oxide nanoparticles encapsulated within nitrogen-doped carbon nanocages for simple and label-free visual detection of blood glucose
Enhanced synergistic effects from multiple iron oxide nanoparticles encapsulated within nitrogen-doped carbon nanocages for simple and label-free visual detection of blood glucose
复制标题
封装在掺氮碳纳米笼内的多种氧化铁纳米颗粒增强了协同效应，可实现简单、无标记的血糖视觉检测
DOI：
10.1088/1361-6528/ab2026
复制
发表时间：
2019-06
期刊：
影响因子：
3.5
通讯作者：
Zhang Min
中科院分区：
文献类型：
--
作者：
Zhong Youquan;Yang Jie;Yin Xiaoying;Zheng Jing;Lu Na;Zhang Min
空心结构碳材料具有比表面积大、孔体积可调、力学性能优异、生物相容性好等优点，在生物传感器、储能、纳米医学等领域的研究中发挥着至关重要的作用。在此，我们开发了一种简单、方便、可控的方法，以二氧化硅纳米球作为牺牲模板，将Fe3O4纳米颗粒包封在空心碳纳米笼（FNHCs）中。由于多个Fe3O4纳米颗粒芯与n掺杂碳纳米笼集成的独特结构，合成的FNHCs表现出极大增强的过氧化物酶模拟活性，具有极高的信噪比（~ 91倍）。此外，FNHCs比其他类似结构的Fe3O4结构（例如Fe3O4@C NPs）具有更高的过氧化物酶样活性。得到的稳态动力学曲线证明了FNHCs的酶活性具有典型的Michaelis-Menton动力学和乒乓机制。基于其优异的酶活性，FNHCs作为一种高效的过氧化物酶模拟物，实现了H2O2和葡萄糖的简便、无标记、高灵敏度/选择性比色检测。此外，该比色传感器成功测定了患者血清样品中的葡萄糖，具有较高的准确度和精密度，具有很大的实际应用潜力。
Hollow-structured carbon materials play a crucial role in research of biosensors, energy storage and nanomedicine as a kind of material with advantages like high surface area, tunable pore volume, excellent mechanical properties, and good biocompatibility. Herein, we developed a simple, facile and controllable method for synthesis of Fe3O4 nanoparticles encapsulated in hollow carbon nanocages (FNHCs) with SiO2 nanospheres as a sacrificial template. Owing to the unique structure of multiple Fe3O4 nanoparticles cores integrated with N-doped carbon nanocages, the as-synthesized FNHCs exhibited greatly enhanced peroxidase mimicking activity with extremely high signal-to-noise ratio of ∼91 fold. Also, it was found that the FNHCs possessed a higher peroxidase-like activity than that of other similar-structured Fe3O4 architectures (e.g. Fe3O4@C NPs). The resulting steady-state kinetic curve demonstrated the enzymatic activity of FNHCs with classic Michaelis–Menton kinetics following a ping-pong mechanism. On the basis of the superior enzymatic activity, the FNHCs performed as a high-efficiency peroxidase mimic, realizing facile, label-free, highly sensitive/selective colorimetric detection of H2O2 and glucose. Furthermore, the colorimetric sensor successfully determined glucose in patients’ serum samples with high accuracy and precision, suggesting great potential for real applications.
登录
查看更多内容
影响因子：
15
作者：
R. Breslow;L. Overman
通讯作者：
R. Breslow;L. Overman
影响因子：
16.6
作者：
Lee, Youngmin;Angel Garcia, Miguel;Sun, Shouheng
通讯作者：
Sun, Shouheng
DOI：
10.1016/j.snb.2018.11.005
发表时间：
2019-03
期刊：
Sensors and Actuators B: Chemical
影响因子：
--
作者：
Min Zhang;Jing Zheng;Jianping Wang;Jingli Xu;T. Hayat;N. S. Alharbi
通讯作者：
Min Zhang;Jing Zheng;Jianping Wang;Jingli Xu;T. Hayat;N. S. Alharbi
影响因子：
16.6
作者：
Wang, Qigang;Yang, Zhimou;Xu, Bing
通讯作者：
Xu, Bing
DOI：
10.1016/s0021-9258(17)44585-6
发表时间：
1983-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
D. J. Porter;H. Bright
通讯作者：
D. J. Porter;H. Bright